枸杞多糖抗溃疡活性部位筛选研究  

Screening of Effective Part of Anti-ulcer Activity from Lyeium barbarum Polysaccharide

作　　者：张一芳[1] 冯怡[2] 徐德生[2] 周淑琴[1] 

机构地区：[1]上海医药高等专科学校,上海201318 [2]上海中医药大学,上海201203

出　　处：《中国实验方剂学杂志》2012年第12期209-211,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:筛选枸杞多糖抗溃疡的活性部位。方法:采用DEAE-纤维素柱层析法将枸杞多糖分成了4个部分(LBP-1,LBP-2,LBP-3,LBP-4),将昆明种小鼠60只按体重性别均衡随机分为6组:模型组,阳性对照组,LBP-1组,LBP-2组,LBP-3组,LBP-4组,采用无水乙醇(10 mL.kg-1)或消炎痛(20 mg.kg-1)溃疡模型对枸杞多糖抗溃疡有效部位进行筛选,给药剂量为阳性对照组100 mg.kg-1,枸杞多糖组250 mg.kg-1,连续给药7 d,每日2次。结果:LBP-2和LBP-4能够显著抑制无水乙醇造成的小鼠胃溃疡(P<0.05),抑制率分别为77.7%,80.1%,LBP-2也能够显著抑制消炎痛造成的小鼠胃溃疡(P<0.05),抑制率为64.7%。结论:LBP-2和LBP-4为枸杞多糖抗溃疡的活性部位,LBP-2的活性最强。Objective： To screen the effective part of anti-ulcer activity from Lyeium barbarum polysaccharide （LBP）. Method： We divided LBP into four parts （LBP-1, LBP-2, LBP-3, LBP-4）by DEAE- cellulose ion exchange chromatography. Sixty mice were randomly divided into six groups： normal control group, positive control group, LBP-1 group, LBP-2 group, LBP-3 group, LBP-4 group. The anti-ulcer components were screened by the experiments of absolute alcohol and indomethacid inducing gastric mucosa ulcer of mice. Positive control group received 100 mg·kg^-1 sucralfate or ranitidine and LBP groups received 250 mg·kg^-1 LBP by ig, twice per day for 7 days. Result： LBP-2 and LBP-4 could inhibitgastric ulcer induced by absolute alcohol significantly （P 〈0.05）, with the inhibition rate of 77.7% and 80.1%. LBP-2 had also the inhibitory effect on gastric ulcer induced by indomethacid （P 〈 0.05） , with the inhibition rate of 64.7%. Conclusion： LBP-2 and LBP-4 can be considered as active parts from LBP, and LBP-2 has a better activity.

关 键 词：枸杞多糖 抗溃疡 活性部位 

分 类 号：R285.5[医药卫生—中药学]