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作 者:张超云[1] 张晓芬[1] 张雪鹏[1] 席先蓉[2]
机构地区:[1]南阳理工学院,河南南阳473004 [2]贵阳中医学院,贵阳550002
出 处:《中国实验方剂学杂志》2012年第12期218-221,共4页Chinese Journal of Experimental Traditional Medical Formulae
基 金:南阳理工学院2009年度科研基金项目(NG2009KYJJ17)
摘 要:目的:初步筛选二陈汤化痰止咳的有效部位。方法:小鼠随机分为12组:生理盐水组、克立停组(DHS)、二陈汤5个提取部位的高、低剂量组(15,3.75 g.kg-1),各组分别ig给药,连续3 d,2次/d,末次给药后1 h,采用氨水引咳法观察不同提取部位对小鼠咳嗽次数的影响;小鼠随机分为12组:生理盐水组、沐舒坦组(AHOS)、二陈汤5个提取部位的高、低剂量组(15,3.75 g.kg-1),各组分别ig给药,连续3 d,2次/d,末次给药后30 min,采用酚红法观察不同提取部位对小鼠气管酚红分泌量的影响;豚鼠随机分为12组:生理盐水组、氨茶碱组(Ami)、二陈汤5个提取部位的高、低剂量组(8.32,2.08 g.kg-1),各组分别ig给药,连续3 d,2次/d,末次给药后1 h,采用组胺诱喘法观察不同提取部位对小鼠翻倒时间的影响。并通过上述方法对5个不同提取部位进行药效学筛选。结果:二陈汤挥发油部位、正丁醇部位和水部位可显著抑制小鼠咳嗽次数、增加小鼠酚红分泌量及延长豚鼠翻倒时间(P<0.01,P<0.05,),而石油醚部位、乙酸乙酯部位对此作用不显著。结论:初步排除石油醚部位、乙酸乙酯部位,并确定二陈汤治疗湿痰证的有效部位为挥发油部位、正丁醇部位和水部位。Objective: To screen the effective parts from Erchen Tang for expectorant and antitussive. Method: The mice were allotted into 12 groups as following: physiogical saline, DHS group, the five part from Erchen decocotion groups with high dose (HD) and low dose (LD, 15, 3.75 g·kg^-1), the mice were orally administered for 3 days, the antitussive effect was observed by concentrated ammonia method after 1 h. The mice were allotted into 12 groups as following: physiogical saline, AHOS group, the five part fron Erchen decocotion groups with high dose (HD) and low dose (LD, 15, 3.75 g·kg^-1), the mice were orally administered for 3 days, the expectorant effect was observed by increased the secretion of expect oration method after 30 min. The cavies were allotted into 12 groups as following: physiogical saline aminophylline group, the five part from Erchen decoction groups with high dose (HD) and 10w dose (LD, 8.32, 2.08 g·kg^-1), the cavies were orally administered for 3 days, the antiasthmatic effect was observed by spray method after 1 h. Result: Essential oil, butanol and water fractions could reduce the frequency of cough, and increase secretion of expectoration, and inhibitory asthma (P 〈 0.01, P 〈 0.05 ) ; petroleum ether and ethyl acetate fractions could not reduce the frequency of cough, could not increase secretion of expectoration, and inhibitory asthma. Conclusion: The results show that petroleum ether and ethyl acetate fractions were the effective parts of Erchen Tang for expectorant and were invalid, essential oil, butanol and water fractions antitussive.
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