中药复方益糖康对糖尿病大鼠NaV1.8蛋白及mRNA表达的影响  

Effect of Chinese Herbal Compound Yitangkang on NaV1.8 Protein and mRNA Expressions of Diabetic Rats

作　　者：刘鹏[1] 马贤德[1] 石岩[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《中华中医药学刊》2012年第6期1291-1292,I0012,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省科技厅资助项目(20082063)

摘　　要：目的:探讨中药复方益糖康对链脲佐菌素(STZ)致糖尿病模型大鼠背根神经节(DRG)中NaV1.8蛋白及mRNA表达的影响。方法:将健康雄性Wistar大鼠40只,体重200-250g,随机分为空白对照组、模型组和益糖康组,其中,模型组和益糖康组采用STZ 55mg/kg腹腔注射复制糖尿病大鼠模型,然后分别给予安慰剂和益糖康5mL.次-1.天-1灌胃,6周后,采集大鼠背根神经节(DRG)标本,用免疫组化和RT-PCR方法分别检测其NaV1.8蛋白和mRNA表达水平。结果:模型组和益糖康组大鼠DRG中NaV1.8蛋白表达灰度值分别为102.19±10.62,134.04±6.56,mRNA表达积分吸光度比值分别为0.219±0.031,0.137±0.014。模型组大鼠DRG中NaV1.8蛋白和mRNA表达水平均显著上调,益糖康组较模型组NaV1.8蛋白和mRNA表达水平均显著降低。结论:中药复方益糖康可能对NaV1.8通道有阻断作用,是其改善糖尿病痛性神经病症状的机制之一。Objective：To explore the effect of Chinese herbal compound Yitangkang on NaV1.8 protein and mRNA expressions of diabetic rats.Methods：40 health male Wistar rats,weighted 200~250g,were randomly divided into blank controlled group,model group and Yitangkang group.Model group and Yitangkang group used STZ 55mg/kg ip to copy diabetes rats model,and were respectively given comfort agent and Yitangkang 5mL/d ig for 6 weeks,rats DRG specimens were collected,with immunohistochemistry and RT-PCR methods were used respectively to detect its NaV1.8 protein and mRNA expression levels.Results：Gray value of NaV1.8 protein expression in model group and Yitangkang group were respectively 102.19±10.62,134.04±6.56,integral optical density ratio of NaV1.8 mRNA expression were respectively 0.219±0.031,0.137±0.014.Results showed that for model group,NaV1.8 protein and mRNA expression in rat DRG increased markedly,compared with the model group,NaV1.8 protein and mRNA expression levels in Yitangkang group were significantly reduced.Conclusion：Chinese herbal compound Yitangkang may be blocking for the NaV1.8 channel,which is proved to be one of the mechanisms of diabetic painful neuropathy symptoms.

关 键 词：糖尿病 痛性神经病 背根神经节 NAV1.8 

分 类 号：R285.5[医药卫生—中药学]