黄芪水煎剂对肾缺血再灌注损伤大鼠肾小管细胞凋亡的影响  

Effect of Astragalus extract on Apoptosis of Tubular Epithelial Cells in Rat Model of Renal Ischemia/reperfusion Injury

作　　者：陈宜方[1] 桂定坤[1,2] 陈建国[1] 黄建华[3] 

机构地区：[1]浙江医院肾内科,浙江杭州310013 [2]上海交通大学附属第六人民医院肾内科,上海200233 [3]复旦大学附属华山医院中西医结合研究所,上海200040

出　　处：《中华中医药学刊》2012年第6期1293-1295,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药管理局基金资助项目(2010ZA001)

摘　　要：目的:探讨黄芪水煎剂(AE)对肾缺血再灌注损伤大鼠肾小管细胞凋亡的影响及机制。方法:雄性SD大鼠随机分为假手术组(Sham)、缺血再灌注损伤组(IR)及IR+不同剂量(0.5、1.0、2.0g/kg)AE预处理组(每组8只),夹闭双侧肾动脉45min再灌注6h后采用原位末端标记法(TUNEL)检测肾小管细胞凋亡,荧光试剂盒检测肾组织Caspase 3活性;实时定量PCR法检测Bcl-2、Bax mRAN表达。结果:与Sham组相比,IR组凋亡细胞数、Caspase 3活性及Bax mRNA表达均显著升高,而Bcl-2 mRNA表达明显降低(P<0.05);AE预处理组凋亡细胞数、Caspase 3活性及Bax mRNA表达均明显减少,而Bcl-2mRAN表达明显升高(P<0.05)。结论:AE能显著抑制IR大鼠肾小管细胞凋亡,机制可能与其调节Bcl-2、Bax表达有关。Objective：To study the effects of Astragalus extract（AE）on apoptosis of tubular epithelial cells in a rat model of renal ischemia/reperfusion（IR）injury and explore its possible mechanisms.Methods：Male Sprague-Dawley rats were randomized into sham operation group,IR group and IR ＋ AE（0.5,1.0,2.0g/kg）treatment group.After clamping bilateral renal pedicles for 45 minutes and allowing reperfusion for 6 hours,the apoptosis of tubular epithelial cells was assessed by terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling（TUNEL）method.Caspase 3 activity was detected by the fluorescent detection system.The mRNA expressions of Bcl-2 and Bax were examined by real-time PCR.Results：Compared with sham group,IR group had significant increase on apoptosis of tubular epithelial cells,Caspase 3 activity and Bax mRNA expression as well as decrease on Bcl-2 mRNA expresion,which was improved by AE pretreatment in a dose-dependent manner.Conclusion：AE inhibited apoptosis of tubular epithelial cells in a rat model of IR injury,which was possibly involved in regulation of Bax/Bcl-2 expression.

关 键 词：黄芪 肾缺血再灌注损伤 细胞凋亡 Bax/Bcl-2基因 

分 类 号：R285.5[医药卫生—中药学]