苓桂术甘汤对心肌梗死后心室重构模型大鼠AngⅡ、Ald和AT_1R的影响  

Effects of Linggui Zhuan Tang on AngⅡ,Ald and AT1R in rats of ventricular remodeling after acute myocardial infarction

作　　者：王靓[1] 侯晓燕[1] 黄金玲[1] 保永亮[1] 施慧[1] 方海雁[1] 程晓煜[1] 

机构地区：[1]安徽中医学院,合肥230038

出　　处：《中国中医基础医学杂志》2012年第6期624-625,628,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金面上项目(30973707);安徽省自然科学基金项目(070413262X);安徽省科技计划项目(10021303024)

摘　　要：目的:观察苓桂术甘汤对心室重构模型大鼠AngⅡ、Ald及AT1R的影响,探讨其干预心室重构的机制。方法:冠脉结扎法复制大鼠模型2周后,各组连续给药4周,采用ELISA法检测血清AngⅡ、Ald含量,采用Western blot法检测心肌组织AT1R表达。结果:模型组与假手术组比较AngⅡ、Ald含量显著升高,AT1R表达显著增加(P<0.01);苓桂术甘汤各剂量组与模型组比较AngⅡ、Ald含量显著降低,AT1R表达显著抑制(P<0.01)。结论:苓桂术甘汤干预心室重构的机制与调控RAAS相关因子有关。Purpose： To study the mechanism of Ling-gui-zhu-gan Decoction interfere ventricular remodeling （VR） of model rats, through observing the effect of Ling-gui-zhu-gan Decoction on the content of angiotensin II （ Ang II）, aldosterone （Aid） and the expression of myocardial tissue angiotensin II type 1 receptor （AT1 R） . Method： 2 weeks after the ligation of coronary artery, each grouB was administered medications intragastrically for 4 consecutive weeks. Detect the content of Ang II and Aid in serum by ELISA and the expression of myocardial tissue AT1R by Western blot. Result： The content of Ang II, Aid and the expression of myocardial ATIR of model group increased significantly compared with sham- operated group （ P 〈 0.01 ）. Low, middle and high dosage of Ling-gui-zhu-gan Decoction could decrease the content of Ang II, Ald in serum of model rats and inhibit the expression of myocardial AT1R significantly compared with model group （P 〈 0.01 ）. Conclusion： The mechanism of Ling-gui-zhu-gan Decoction interferes VR seems to be related to the regulation of the correlating factor of rennin angiotensin aldosterone system.

关 键 词：苓桂术甘汤 心室重构 血管紧张素Ⅱ 醛固酮 血管紧张素Ⅱ受体1 

分 类 号：R285.5[医药卫生—中药学]