海南山苦茶提取物对衰老小鼠脑组织抗氧化能力的影响  

Effect of leave extract of Mallotus furetianus cultivated in Hainan Island on anti-oxidation capacity in brain tissue of aging mice

作　　者：刘月丽[1] 黄奕弟[2] 林连波[1] 

机构地区：[1]海南医学院药学院,海南海口571199 [2]海南医学院理学院,海南海口571199

出　　处：《海南医学院学报》2012年第7期872-874,共3页Journal of Hainan Medical University

基　　金：国家自然科学基金(30060088)~~

摘　　要：目的:研究海南山苦茶提取物对衰老小鼠脑组织抗氧化能力的影响。方法:120只小鼠随机分为正常组、模型组、阳性药组(维生素E)及海南山苦茶高、中、低剂量组,除正常组外,其余各组小鼠颈背部皮下注射D-半乳糖120mg/kg,连续8周,诱导衰老模型;同时,阳性药组小鼠灌胃给予维生素E 50mg/kg,海南山苦茶高、中、低剂量组小鼠分别灌胃给予81、57、40mg/kg海南山苦茶提取物,正常组和模型组小鼠灌胃给予等体积的0.5%羧甲基纤维素钠。8周后,处死小鼠,取脑组织,匀浆,测定脑组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-px)活性以及丙二醛(MDA)的含量。结果:模型组小鼠MDA含量较正常组明显升高(P<0.01),SOD、CAT、GSH-px活性较正常组明显降低(P<0.01);维生素E组和山苦茶提取物高、中、低剂量组小鼠的MDA含量较模型组明显降低(P<0.01),CAT活性较模型组明显升高(P<0.01),山苦茶提取物高、中剂量组SOD和GSH-px活性较模型组明显升高(P<0.01)。结论:海南山苦茶提取物能够提高衰老小鼠脑组织的抗氧化能力。Objective. To study effect of leave extract of Mallotusfuretianus cultivated in Hainan Island on anti-oxidation capacity in brain tissue of aging mice. Methods：Aging mice model was established by injecting D-galactase 120 mg/kg subcutaneously, leave extract of Mallotusfuretianus was given at dif- ferent concentration. After 8 weeks, the mice were sacrificed to investigate activities of superoxide dis- mutase （SOD）, catalase （CAT）, glutathione peroxidase （GSH-px） and the amount of malonaldehyde （MDA） in brain tissue. Results. Leave extract of Mallotus furetianus could increase activities of SOD, CAT, GSH-px and reduce amount of MDA. Conclusions- Leave extract of Mallotusfuretianus can enhance anti-oxidation capacity in brain tissue of aging mice.

关 键 词：山苦茶 提取物 衰老 抗氧化 

分 类 号：R917[医药卫生—药物分析学]