HPLC法测定消炎利胆冲剂中大黄酸、大黄素的含量  

Determination of Rhein and Emodin in XiaoYan LiDan Granule with HPLC

作　　者：白梓静[1] 齐广才[1] 刘珍叶[1] 

机构地区：[1]延安大学化学与化工学院,陕西延安716000

出　　处：《西部中医药》2012年第6期15-17,共3页Western Journal of Traditional Chinese Medicine

摘　　要：目的:采用高效液相色谱法测定消炎利胆冲剂中大黄酸、大黄素的含量。方法:以甲醇-0.2%冰醋酸溶液(80∶20)作为流动相,色谱柱为Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm),流速为1.0 mL/min,柱温为28℃,检测波长为254 nm。结果:大黄酸、大黄素的线性范围分别为:0.054～180μg/mL、0.0612～204μg/mL。结论:本方法灵敏度高、选择性好、操作简便,可用于消炎利胆冲剂的质量控制。Objective： To determine contents of rhein and emodin in XiaoYan LiDan granule with HPLC. Method： The mobile phase was methanol-0.2% acetic acid （80：20）, the column was Agilent ZORBAX SB-C18 （4.6 mm×250 mm, 5μm）, the flow rate was 1.0 ml/min, the column temperature was 28℃, detection wavelength was 254nm. Result： There were good linear relationships of rhein and emodin in the range of 0.054-180 μg/mL and 0.0612-204μg/mL. Conclusion： The method, with the advantages of high sensitivity, good selectivity and simple operation, could be used in quality control ofXiaoYan LiDan granule.

关 键 词：消炎利胆冲剂 大黄酸 大黄素 高效液相色谱法 

分 类 号：R289[医药卫生—方剂学]