Study of Krypton Laser-Induced Choroidal Neovascularization in a Guinea Pig Model of High Anisometropia  

作　　者：Jianghui Wang Wei Jiang Zefeng Kang Lina Liang Xiaoman Liu Nannan Tian Qing Zhang 

机构地区：[1]Medical College of Qinghai University, Qinghai 810007, China [2]Eye Hospital of China Academy of Traditional Chinese Medical Sciences, Beijing 100040, China

出　　处：《Eye Science》2012年第2期76-81,84,共7页眼科学报（英文版）

基　　金：National Natural Science Foundation(grant No.81072843)

摘　　要：Purpose:To investigate the association between high anisometropia and the area of choroidal neovascularization (CNV) induced by krypton laser in guinea pigs and better understand the pathogenesis and prevention of myopic CNV. Methods:.Nine 3-week old male guinea pigs with anisometropia > 6.00D were randomly assigned to three groups according to examination date after laser photocoagulation (7d, 14d and 28d). All animals underwent refraction. The eye with higher myopia was used as the experimental eye, and the other as the control eye..All eyes received repeated multi-wavelength krypton laser photocoagulation treatments (wavelength: 532 nm; laser power: 400 mW; spot diameter: 50 μm; exposure time: 0.1s). Fundus photography and indocyanine green angiography (ICGA) were performed. Afterwards, the animals were sacrificed immediately,.and the eyes were enucleated and processed for histopathologic examination and flat mounts. Results: CNV appeared at 7d after laser treatment. The area of CNV peaked at 14d, and decrease in area and the presence of scarring was noted at 28 d..CNV was present in 66.7% of eyes by ICGA at 14 d..CNV could be observed under light microscopy at all three time points..At 14 d,.flat mount showed the neovascular plexus around the lesion. Semi-quantitative analysis revealed that the area of CNV in treated eyes was greater than that of control eyes. Conclusion:.Since the mechanism of CNV in this study resembles that of CNV in pathological myopia, this model can be used to investigate the etiology, pathogenesis and treatment of CNV in pathological myopia.Purpose： To investigate the association between high ani- sometropia and the area of choroidal neovascularization （CNV） induced by krypton laser in guinea pigs and better un- derstand the pathogenesis and prevention of myopic CNV. Methods： Nine 3-week old male guinea pigs with ani- sometropia 〉 6.00D were randomly assigned to three groups according to examination date after laser photocoagulation （7d, 14d and 28d）. All animals underwent refraction. The eye with higher myopia was used as the experimental eye, and the other as the control eye. All eyes received repeated multi- wavelength krypton laser photocoagulation treatments （wave- length： 532 nm; laser power： 400 mW; spot diameter： 50 /xm; exposure time： 0.1s）. Fundus photography and indocya- nine green angiography （ICGA） were performed. Afterwards, the animals were sacrificed immediately, and the eyes were enucleated and processed for histopathologic examination and fiat mounts. Results： CNV appeared at 7d after laser treatment. The area of CNV peaked at 14d, and decrease in area and the presence of scarfing was noted at 28 d. CNV was present in 66.7% of eyes by ICGA at 14 d. CNV could be observed under light microscopy at all three time points. At 14 d, flat mount showed the neovascular plexus around the lesion. Semi-quantitative analysis revealed that the area of CNV in treated eyes was greater than that of control eyes. Conclusion： Since the mechanism of CNV in this study re- sembles that of CNV in pathological myopia, this model can be used to investigate the etiology, pathogenesis and treatment of CNV in pathological myopia.

关 键 词：新生血管 激光诱导 脉络膜 豚鼠 模型 病理组织学检查 激光治疗 氪 

分 类 号：S966.35[农业科学—水产养殖] Q78[农业科学—水产科学]