时间分辨荧光免疫法在乙肝标志物检测中的应用价值  

Clinical application value of time-resolved fluorescence immunoassay for detection of serum markers of hepatitis B virus

作　　者：谭业克[1] 

机构地区：[1]泰安市中心医院,山东泰安271000

出　　处：《基层医学论坛》2012年第16期2071-2072,共2页The Medical Forum

摘　　要：目的探讨时间分辨荧光免疫分析(TRFIA)检测乙肝病毒血清标志物(HBV-M)的临床价值。方法用酶联免疫吸附试验(ELISA)和时间分辨荧光免疫分析分别对460例血清标本进行乙肝标志物的定性检测和比较,结果不符的用电化学发光仪进行复检,对结果进行分析。结果两种方法检测乙肝病毒标志物无显著差异,但TRFIA提高了病毒早期感染的检出率,表面抗体的效价定量便于把握疫苗的接种时机,有效降低了假阳性和假阴性的发生率。结论 TRFIA法定量检测乙肝标志物的浓度变化与常规ELISA法相比较,灵敏度更高,特异性更好,对乙型肝炎的疾病进程具有全程动态检测的作用,能够帮助医生对治疗效果作出有效的判断。Objective To study the clinical application value of time-resolved fluorescence immunoassay for detection of serum markers of hepatitis B virus. Methods Using enzyme immunoassay （ELISA） and time-resolved fluorescence immunoassay （TRFIA） respectively in 460 serum specimens , hepatitis B markers were qualitatively detected and compared. The inconsistent results were tested again with Electrochemical luminescence instrument, analysis of the results. Results Two methods for the detection of hepatitis B virus markers does not exist significant difference, but TRFIA improve the early detection rate of infection, Surface antibody quantitative analysis can grasp the timing of vaccination, lower false positive and false negative rate, the method is worth popularizing. Conclusions Time-resolved fluorescence immunoassay for detection of serum markers of hepatitis B virus had good sensitivity and good specificity andit is helpful to estimate the effect.

关 键 词：乙肝病毒标志物 酶联免疫吸附法 时间分辨荧光免疫法 临床应用 

分 类 号：R446.6[医药卫生—诊断学]