葡萄SVP类MADS-box基因的克隆及表达分析  

Cloning and Expression Analysis of Grape SVP-like MADS-box Gene

作　　者：杨堃[1] 张朝红[1] 李树秀[1] 张旭彤[1] 王跃进[1] 

机构地区：[1]西北农林科技大学园艺学院农业部西北地区园艺作物生物学与种质创制重点实验室旱区作物逆境生物学国家重点实验室,陕西杨陵712100

出　　处：《西北林学院学报》2012年第4期117-123,共7页Journal of Northwest Forestry University

基　　金：国家自然科学基金项目(31171926);国家现代农业产业技术体系建设专项基金(CARS-30-yz-7);公益性行业(农业)科研专项(200903044)

摘　　要：从无核白葡萄(Vitis vinifera cv.Thompson seedless)中克隆获得一个胚珠发育相关MADS-box基因。该基因cDNA全长1 248bp,ORF为729bp,编码一个含有243个氨基酸的蛋白。氨基酸多序列比对和系统发育分析显示,该基因属于SVP类MADS-box基因。采用半定量技术进行表达分析表明,该基因在葡萄的根、茎、叶、花蕾、盛花和胚珠中均有表达,但是有强弱差异。实时定量PCR技术分析表明在无核葡萄品种无核白胚珠发育过程中基因表达量先升后降,而在有核葡萄品种黑比诺胚珠发育过程中表达量一直呈下降趋势。A MADS-box gene Thompson seedless. The full-1 and encoding 243 amino acids. SVP-like MADS-box genes. T associated with ovule development was cloned from Vitis. vinifera cv. ength of cDNA gene was 1 248 bp, including a 729 bp open reading frame, The homology and phylogenetic analysis showed that this gene belonged to he gene was expressed in all of the grape tissue type examined by semi-quan- tity RT-PCR, including root, stem, leaf, bud, full bloom and ovules, but varied widely in the level of ex- pression. Real-time PCR analysis revealed that gene expression level during ovule development in Thomp- son increased firstly and then decreased. In contrast to this, gene expression level in Pinot showed down- ward trend in all stages of development.

关 键 词：葡萄 SVP类MADS—Box基因 表达分析 

分 类 号：S663.1[农业科学—果树学]