51例新生儿脐带血单纯疱疹病毒的检测  

Detection of herpes simplex virus infection in umbilical cord blood in 51 neonates

作　　者：任鹏[1] 姚娟[1] 金艳[2] 李法锦[1] 汪晓婷[1] 葛俊[1] 江龙凤[1] 赵蕾[1] 吉阳[1] 王明丽[1] 

机构地区：[1]安徽医科大学微生物学教研室,合肥230032 [2]合肥市妇幼保健院,合肥230001

出　　处：《微生物与感染》2012年第2期95-98,共4页Journal of Microbes and Infections

基　　金：国家自然科学基金(30872253);安徽省科技厅科技攻关项目(08010302179)

摘　　要：为了解健康产妇顺产新生儿单纯疱疹病毒(HSV)感染情况,2010年11月～2011年6月于某地妇幼保健院采集顺产新生儿抗凝脐带血51份。提取新生儿脐带血单核细胞DNA后,利用HSV-1gD基因和HSV-2gG基因特异性引物,进行套式聚合酶链反应(PCR)检测,并将阳性扩增产物测序后进行BLAST比对确认,分析HSV感染阳性率。结果显示,51份脐带血HSV-1阳性12份,感染率23.53%;HSV-2阳性13份,感染率25.49%;HSV-1和HSV-2共阳性4份,共感染率7.84%。结果表明,加强新生儿HSV感染的早期诊断和治疗,对预防新生儿HSV感染所致疾病、防止后遗症具有重要意义。The present paper aims to investigate the herpes simplex virus （HSV） infection in neonates. From November 2010 to June 2011, fresh neonatal cord blood was collected from 51 neonates in a Maternity and Child Hospital and transported immediately to our laboratory. After DNA extraction from the samples, nested-polymerase chain reaction （PCR） was performed by using HSV-1 gD gene- and HSV-2 gG gene- specific primers to detect congenital HSV-1 and HSV-2 infections respectively. The PCR products were sequenced to confirm the HSV infections. The results indicated that the infection rate of HSV-1 and HSV-2 were 23.53% and 25.49% respectively. The HSV-1/HSV-2 co-infection rate was 7.84%. This study suggests that early diagnosis and treatment of neonatal HSV infection is critical for the prevention of diseases caused by HSV infection.

关 键 词：单纯疱疹病毒感染 脐带血 新生儿 

分 类 号：R722.1[医药卫生—儿科]