白念珠菌与LDH抗体的交叉免疫研究  

Cross-reactive immune response of Candida albicanswith anti-LDH antibody

作　　者：钟毅[1] 黄怀球[1] 赵静[1] 袁立燕[1] 张静[2] 张晓辉[3] 李美荣[1] 

机构地区：[1]中山大学附属第三医院皮肤性病科,广州510630 [2]中山大学附属孙逸仙纪念医院皮肤性病科,广州510120 [3]广东省皮肤性病防治中心,广州510500

出　　处：《中国人兽共患病学报》2012年第6期612-615,共4页Chinese Journal of Zoonoses

基　　金：广东省科技计划项目(No.2011B031800277)~~

摘　　要：目的识别白念珠菌苹果酸脱氢酶(Candida albicans malate dehydrogenase,CaMDH)保守表位结构。方法本研究利用生物信息学方法,预测CaMDH的结构、功能和进化关系,并选取同源序列中与CaMDH序列一致性较低的日本血吸虫乳酸脱氢酶(Schistosoma japonicum lactate dehydrogenase,SjLDH)免疫血清与白念珠菌总蛋白进行交叉免疫反应。结果发现CaMDH与常见病原生物的同源序列一致性可达43%,其中与SjLDH一致性最低(18.2%)。CaMDH具有乳酸脱氢酶保守区域,7个主要的B细胞表位,其中3处与SjLDH表位相似。苹果酸脱氢酶活化点包含于NAD(P)结合部分中。白念珠菌总蛋白与SjLDH免疫血清交叉免疫反应阳性。结论交叉免疫反应阳性提示识别结合位点为LDH/MDH同源序列高度保守的表位结构,为进一步从高度保守的抗原决定簇中筛选真菌疫苗表位、诊断特异抗体、抗真菌药物治疗靶点提供了实验基础。To identify the conserved epitopes of Candida albicans malate dehydrogenase（CaMDH）,the structure,function and phylogenetic analysis of the CaMDH sequence were predicted by using tools of bioinformatics.The phylogenetic analysis showed the amino acid sequence of Schistosoma japonicum lactic dehydrogenase（SjLDH） had lowest identity（18.2%） with CaMDH than that of other species（43%）.The structure prediction result showed that CaMDH contained LDH conserved domain and 7 main B cell epitopes,in which 3 epitopes displayed high levels of similarity with that of SjLDH.The MDH active site located at the NAD（P） binding domain.Furthermore,the SjLDH recombinant protein immune serum which had lowest identity with CaMDH in study was chosen to make cross-reactive immune response with Candida albicans protein.The positive result with the anti-LDH body identify that the interaction point was highly conserved epitopes of CaMDH homologous sequence and supply new methods for the vaccine,diagnosis and drug target study of the CaMDH.

关 键 词：白念珠菌 苹果酸脱氢酶 乳酸脱氢酶抗体 交叉免疫 

分 类 号：R379.9[医药卫生—病原生物学]