大鼠肠神经系统内γ-氨基丁酸能神经元与乙酰胆碱、一氧化氮、血管活性肠肽的共存关系  

THE COLOCALIZED RELATIONSHIPS BETWEEN GABA AND ACETYLCHOLINE,NITRIC OXIDE,VASOACTIVE INTESTINAL PEPTIDE IN ENTERIC NERVOUS SYSTEM OF THE RAT

作　　者：陈鹏[1] 胡立华[1] 苏连明[2] 石春林[1] 张沛怡[1] 

机构地区：[1]黑龙江省医院,黑龙江哈尔滨150036 [2]牡丹江医学院附属红旗医院,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2012年第3期12-15,共4页Journal of Mudanjiang Medical University

基　　金：黑龙江省卫生厅科研项目(2006-432)

摘　　要：目的:探讨-氨基丁酸(GABA)在大鼠肠神经系统中与乙酰胆碱、一氧化氮、血管活性肠肽的共存关系。方法:应用免疫组织化学和免疫荧光双重染色技术对大鼠肠神经系统内谷氨酸脱羧酶(GAD)与乙酰胆碱酯酶(AchE)、一氧化氮合酶(NOS)、血管活性肠肽(VIP)的共存关系进行观察。结果:GAD样阳性神经元主要位于肌间神经丛,与AchE、NOS、VIP存在共存关系。双重标记神经元分别占GAD阳性神经元的5.6%、62.6%和51.1%,而占AchE、NOS、VIP阳性神经元的3.4%、19.8%和43.5%。结论:在肠神经系统中GABA可能通过调节其它神经递质的释放而发挥着间接的调控作用。Objective：To explore the possible colocalized relationships between GABA and acetylcholine, nitric oxide, vasoactive intestinal peptide in enteric nervous system （ENS） of the rat. Methods： The coexistent neurons of glutamic acid decarboxylase （GAD） with acetylcholinesterase （AchE）, nitric oxide synthase （NOS） and vasoactive intestinal peptide （VIP） in enteric nervous system was observed under microscope by means of immunohistochemistry and immunofluorescence histochemical double - staining methods. Re~ suits ： GAD - IR neurons were mainly located in the myenteric plexus, some GAD - IR neurons also showed AchE - IR, NOS - IR or VIP - IR. 5.6%, 62.6%, 51.1% of GAD - IR nerve cells were double staining neurons respectively, while 3.4% of AchE - IR, 19.8% NOS - IR and 43.5% VIP - IR neurons also showed GAD - IR. Conclusion：The present results indicated that GABA may play an indirect influence through the regulation of other neurotransmitters release in ENS.

关 键 词：肠神经系统 -氨基丁酸 乙酰胆碱 一氧化氮 血管活性肠肽 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]