胫骨癌痛大鼠痛觉敏化的形成及其脊髓机制  

Hyperalgesia in rats with tibial cancer pain: formation and possible mechanisms

作　　者：赵倩倩[1] 杜其航[1] 王守靓[1] 王公明[1] 张孟元[1] 

机构地区：[1]山东大学附属省立医院麻醉科,济南250021

出　　处：《山东大学学报（医学版）》2012年第7期37-40,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(30872433)

摘　　要：目的探讨胫骨上段骨髓腔接种MADB-106大鼠乳腺癌细胞后,大鼠痛觉敏化的形成及其脊髓机制。方法雌性SD大鼠16只,按照随机数字表法分为正常对照组(C组)和模型组(M组),每组8只。M组大鼠胫骨上段骨髓腔接种MADB-106大鼠乳腺癌细胞,C组骨髓腔注入10μL 0.9%氯化钠溶液,其余同M组。分别于接种前1天及接种后8、15、21 d,称体质量,记录自发性缩足反射次数及自由活动时下肢跛行程度。并于接种后第8、15、21天对大鼠接种侧和对照侧后肢行X线检查。接种后22 d采用HE染色法观察癌细胞对骨结构的破坏程度,采用免疫荧光检测脊髓背角GFAP蛋白的表达情况。结果 M组大鼠术后15 d起体质量增长慢于C组(P<0.05或0.01);M组术后第8天起自主缩足次数比C组明显增加,差异有统计学意义(P<0.05或0.01);术后第8天起自由行走痛评分比C组有所增加,差异有统计学意义(P<0.05或0.01)。胫骨X线检查显示明显的骨破坏。骨组织HE染色显示骨髓腔内肿瘤生长,向外侵蚀破坏骨皮质。免疫荧光染色显示术侧脊髓背角星形胶质细胞大量激活。结论大鼠胫骨骨髓腔接种MADB-106乳腺癌细胞2周后痛觉敏化形成,这可能与脊髓背角星型胶质细胞的激活有关。Objective To study hyperalgesia formation and its possible mechanisms in rats after inoculation of MADB- 106 mammary gland carcinoma cells. Methods 16 female SD rats were randomly divided into 2 groups. MADB-106 mammary gland carcinoma cells were inoculated into the marrow cavity of SD rats＇ tibia （the model group, or M Group; 8 rats）. The control group （C Group） also had 8 rats. The frequency of spontaneous paw withdrawal was doc- umented and moving-evoked pain was recorded by observing the extent of hind limb claudication when the rats walked. Structural damage of the bone was monitored by X-ray and histological analyses. Expression of GFAP in spinal astro- cytes were investigated with immunofluorescence histochemistry. Results Growth of rats was much slower in the M Group than in the C Group , as observed 15 d post-inoculation. The frequency of spontaneous paw withdrawal was much higher in the M Group than in the C Group 8 d post-inoculation. The score for moving-evoked pain for the M Group was significantly higher than the C Group 8 d post-inoculation. X-ray of the tibia showed servere bone destruction in the M Group; this was confirmed by a histological analysis which reavealed that the bone marrow cavity was full of tumor cells and the cortical bone was destroyed. Immunofluorescence histochemistry showed that the spinal astrocytes were activated, with strong GFAP expression. Conclusion Hyperalgesia in rats was developed two weeks after inocu- lation with MADB-106 mammary gland carcinoma cells, which is associated with the activation of spinal astrocytes.

关 键 词：骨癌痛 动物模型 胫骨 接种 痛敏 

分 类 号：R441.1[医药卫生—诊断学]