SIVmac239感染恒河猴急性期回肠派氏淋巴结中淋巴细胞亚群的变化  

The Changes of Lymphocytes from Ileum Peyers Pacth from Rhesus Monkeys at the Earliest Period with SIVmac239 Infection

作　　者：刘克剑[1] 丛喆[1] 吴芳新[1] 王卫[1] 熊竞[1] 魏强[1] 秦川[1] 

机构地区：[1]中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021

出　　处：《中国比较医学杂志》2012年第6期25-29,87,共6页Chinese Journal of Comparative Medicine

基　　金：“十二五”国家科技重大专项课题(2012ZX10004-501);协和青年基金(SIV感染早期粘膜屏障结构的改变)

摘　　要：目的分析SIVmac239感染早期中国恒河猴回肠派氏淋巴结淋巴细胞数量及亚群的变化,探讨这些变化与疾病进展的可能关系。方法以静脉注射SIVmac239制备恒河猴AIDS模型,对回肠派氏淋巴结进行CD4和CD8免疫组化标记,分离Peyer’s集合淋巴结淋巴细胞,分别标记CD3、CD4、CD8、CD28、CD95单克隆抗体,以流式细胞仪检测T细胞及其亚群的表达情况。结果 SIVmac239感染急性期中国恒河猴Peyer淋巴结中CD4+/CD8+比值持续下降,记忆性细胞比例升高,但Peyer淋巴结形态及CD4+T细胞数量未见明显变化,CD8+T细胞从第5天开始持续升高。结论 SIVmac239感染急性期,中国恒河猴回肠派氏淋巴结形态及CD4+T细胞数量基本维持,向记忆性细胞的转化增加,但是CD4+/CD8+比值下降。Objective To analyze the changes of lymphocytes from ileum Peyers pacth from Chinese rhesus monkeys at acute period with SIVmac239 infection.Methods 9 rhesus monkeys were used and 5 were infected with SIVmac239 via intravenous injection.The tissues or lymphocytes from ileum Peyers pacth lymph nodes from rhesus monkeys at acute period were collected and analyszed with CD4＋,CD8 immunohistochemistry and flow cytometry T-cell subsets labeling with CD3,CD4,CD8,CD28,CD95 monoclonal antibodies respectively.Results Obviously declined CD4＋/CD8＋ ratio and increased memory cells（CD4 ＋ CD8 ＋） ratio and CD8＋ T cells were detected in Peyers lymph nodes,but CD4 counts had no significant change in Peyers lymph nodes.Conclusion Declined CD4＋/CD8＋ ratio and increased memory cells（CD4 ＋ CD8 ＋） ratio were found in Peyers lymph nodes from rhesus macaques at acute period with SIVmac239 infection,however,the meaning and possible affection to disease need further study.

关 键 词：AIDS 派氏淋巴结 T淋巴细胞 恒河猴 

分 类 号：R373.33[医药卫生—病原生物学] R332[医药卫生—基础医学]