人参总皂苷和远志总苷配伍对小鼠抗抑郁作用  

Study of Antidepressant-like Effects of Combination of Ginseng Total Saponins and Polygala Tenuifolia Total Glycosiders in Mice

作　　者：孙秀萍[1,2] 李腾飞[1,3] 石哲[1] 刘进修[1] 潘瑞乐[1] 王立为[1] 刘新民[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]山东中医药大学药理教研室,济南250355 [3]广西医科大学,南宁530021

出　　处：《中国比较医学杂志》2012年第6期30-36,共7页Chinese Journal of Comparative Medicine

基　　金：国家科技重大专项课题-重大新药创制项目(2009ZX09103-336;2009ZX09502-014);对欧科技合作专项(0901)

摘　　要：目的确定人参总皂苷(GTS)和远志总苷(PTG)抗抑郁配伍剂量比例,形成参远苷(SGY)制剂,为研制开发抗抑郁新药提供实验数据。方法采用析因设计方法,GTS和PTG均选取25、50、100 mg/kg三个剂量,按照完全随机的两因素3×3实验设计,得到参远苷的9个不同配比组。C57BL/6J小鼠(用于悬尾实验)和ICR小鼠(用于强迫游泳实验)随机分为对照组、阳性药组(10 mg/kg,帕罗西汀用于悬尾实验;阿米替林用于强迫游泳实验)及参远苷的9个不同配比组,共11组。灌胃给药7 d,观察各组对悬尾或强迫游泳实验小鼠不动时间的影响,并通过空场实验观察参远苷各配比对小鼠自主活动的影响。参远苷与单味GTS、PTG的抗抑郁作用比较实验中,C57BL/6J小鼠(用于悬尾实验)和ICR小鼠(用于强迫游泳实验)随机分为对照组、阳性药组(10 mg/kg,帕罗西汀用于悬尾实验;阿米替林用于强迫游泳实验)、参远苷低中高剂量组(37.5、75、150 mg/kg)、GTS和PTG各四个剂量组(均为18.75、37.5、75、150 mg/kg),共13组。灌胃给药7 d,观察各组对悬尾或强迫游泳实验小鼠不动时间的影响。结果析因设计结果表明,GTS和PTG之间无交互效应。参远苷配比组75 mg/kg(GTS∶PTG为50∶25)及150mg.kg-1(GTS∶PTG为100∶50)显著并稳定缩短悬尾或强迫游泳不动时间(P<0.05),得出GTS和PTG的剂量配伍比例为2:1。空场实验结果显示,参远苷各配比对小鼠运动总路程无影响。参远苷与单味GTS、PTG抗抑郁作用比较实验结果显示,GTS 75、150 mg/kg缩短悬尾实验小鼠不动时间(P<0.01,P<0.05),对强迫游泳实验小鼠不动时间无影响。PTG 18.75、37.5 mg/kg缩短强迫游泳实验小鼠不动时间(P<0.01,P<0.05),对悬尾实验小鼠不动时间无影响。参远苷75、150 mg/kg缩短悬尾实验小鼠不动时间(P<0.05)。同时,参远苷37.5、75 mg/kg缩短强迫游泳实验小鼠不动时间(P<0.01,P<0.05)。结论 GTS和PTG以2:1的比例形成的参远苷(SYObjective To investigate the antidepressant-like effects of combination of ginseng total saponins（GTS） and polygala tenuifolia total glycosiders（PTG） in mice.Method The factorial design analysis of variance（ANOVA） was employed to investigate the appropriate combination of GTS and PTG.The forced swim and tail suspension test were performed to evaluate the efficacy of SYG.Open field test was used to assess the psycho-stimulant side-effects.C57BL/6J mice（for tail suspension test） and ICR mice（for forced swim test） were randomly separated into 11 groups（n = 10）： control（distilled water）,positive control（10 mg/kg,paroxetine for TST,amitriptyline for FST）,or treated with SYG（nine groups）.All drugs and water were administered by gastric gavages（20 mL/kg） once daily for seven days.The behavioural tests in mice were conducted on the 7th day after the last administration.To compare the antidepressant effects of SYG,GTS and PTG,C57BL/6J mice（for tail suspension test） and ICR mice（for forced swim test） were randomly separated into 13 groups（n = 10）： control（distilled water）,positive control（10 mg/kg,paroxetine for TST,amitriptyline for FST）,or treated with SYG（37.5,75,150 mg/kg）,GTS,PTG（18.75,37.5,75,150 mg/kg）.All drugs and water were administered by gastric gavages（20 mL/kg） once daily for seven days.The behavioural tests in mice were conducted on the 7th day after the last administration.Results In the factorial design,SYG reduced the immobility time in the tail susupension and forced swim test at doses of 75 mg/kg（GTS∶PTG = 50∶25） and 150 mg/kg（GTS∶PTG = 100∶50）（P 0.05）.Accordingly,the appropriate combination of GTS and PTG was 2∶1.SYG did not produce significant effects on the locomotor activity in the open-field test.In the subsequent test,GTS reduced the immobility time in the tail suspension test at doses of 75,150 mg/kg（P 0.01,P 0.05）,but did not reduce the immobility time in the forced swim test.PTG reduced the imm

关 键 词：人参 远志 抗抑郁 悬尾 强迫游泳 析因设计 

分 类 号：R332[医药卫生—人体生理学]