STZ诱导恒河猴糖尿病动物模型的建立  

Establishment and Evaluation of Experimental Rhesus Monkeys Diabetes Model

作　　者：朱华[1] 刘颖[1] 马春梅[1] 黄澜[1] 秦川[1] 

机构地区：[1]卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京100021

出　　处：《中国比较医学杂志》2012年第6期53-56,共4页Chinese Journal of Comparative Medicine

基　　金：863课题(2009ZX09503-025)

摘　　要：目的复制稳定的链脲佐菌素诱导糖尿病恒河猴动物模型。方法健康恒河猴5只,小剂量(30mg/kg)多次静脉注射链脲佐菌素,分别在注射后2、3个月进行葡萄糖耐量实验并连续观察血糖、胰岛素、C肽的变化。连续观察12个月。结果随着时间推移,动物出现典型的糖尿病症状。3只动物的血糖静脉注射1次后持续10周稳定,另外2只分别进行了第2、3次注射。动物血糖在12个月内平稳上升、胰岛素、C肽分泌持续下降。2个月时葡萄糖耐量减低明显(P<0.01)。结论小剂量多次注射STZ后恒河猴可出现持续、稳定的糖尿病表现,可作为相关研究的动物模型。Objective To make a reproducible diabetes mellitus model in macaques by injecting streptozotocin（STZ）.Methods we used STZ（30 mg/kg of body weight,intravenously,3days） to five macaques.The dynamic changes of the glucose,C-peptide and insulin contents in plasma were measured for 12 months.The IVGTT were made at 2 and 3 month.Results Three of five monkeys became markedly hyperglycemic,2 other monkeys received a second and third dose of STZ intravenously（30 mg/kg） after the first dose.Compared with the contents before injection,the glucose contents increased significantly while C-peptide and insulin contents reduced in plasma in macaques 1 week after streptozotocin injection（P0.01）,Diabetic monkeys also had abnormal responses to an intravenous glucose tolerance test at 2 and 3month.Conclusion The results showed that low dose with 30 mg/kg STZ could lead to the significant changes in glucose,C-peptide and insulin content stably,indicating that macaques diabetes mellitus model was established successfully.

关 键 词：糖尿病 模型 动物 恒河猴 链脲佐菌素 

分 类 号：R332[医药卫生—人体生理学]