β-连环素在人颈椎终板软骨中的表达及其意义  

Significance and Expression of β-catenin in Human Cervical Cartilaginous Endplate

作　　者：宋俊兴[1] 徐宏光[1] 章平治[1] 李梓瑞[1] 程加峰[1] 吕坤[2] 钟民[2] 张梦莹[2] 所起凤[2] 

机构地区：[1]皖南医学院附属弋矶山医院脊柱外科,安徽芜湖241001 [2]皖南医学院附属弋矶山医院中心实验室

出　　处：《解剖与临床》2012年第3期201-205,共5页Anatomy and Clinics

基　　金：国家自然科学基金（30973025）、卫生部公益性行业专项基金（201002018）、安徽省教育厅自然科学研究基金重点项目（KJ2010A320）

摘　　要：目的：观察β-连环素在人颈椎椎体终板软骨中的表达变化，探讨其与终板软骨退变的关系及意义。方法：选取2011年9月至2012年3月间住院接受颈前路椎体次全切手术的颈椎终板软骨标本，分为退变组（28例）和对照组（10例），运用RT—PCR和western—blot法检测终板软骨组织中β-连环素、蛋白多糖、Ⅱ型胶原及Sox9基因的表达。结果：退变组和对照组均可检测到β-连环素的表达，而退变组β-连环素表达量明显高于对照组（P〈0．01），蛋白多糖、Ⅱ型胶原及Sox9基因表达量明显低于对照组（P〈0．01）。结论：人退变颈椎终板软骨组织内β-连环素表达明显升高，提示其与椎体终板软骨退变存在重要关系。Objective：To observe the expression of β-catenin in normal and degenerative cervical verte bral cartilaginous endplate, and investigate the role of β-catenin in the progress of degeneration of human cervi cal cartilaginous endplates. Methods：The cervical cartilaginous endplates of 38 patients who performed surgical treatment from Sep. 2011 to Mar. 2012 were divided into control group （ n = 10） and degeneration group （ n = 28）. β-catenin ,aggrecan, type Ⅱ collagen and Sox9 were detected by RT PCR and western blot. Results： β-eatenin was detected by all cervical cartilaginous endplates. Compared with the control group, [3catenin ex pression of degeneration group significantly increased （ P 〈 0.01 ） , and the expression level of aggrecan , typeⅡ collagen and Sox9 gene markedly decreased （ P 〈 0. Ol ）. Conclusions： β-catenin expression significantly in creases in the degeneration of cartilaginous endplate, it has an important relationship between the human cervical vertebral cartilaginous endplate.

关 键 词：椎间盘退变 Β-连环素 软骨终板 WNT 软骨表型 

分 类 号：R687.3[医药卫生—骨科学]