塔里木马鹿腔前卵泡分离方法比较  

The Analysis on Isolation Method of Cervus elaphus yarkandensis Preantral Follicles

作　　者：汪建红[1] 陈静波[2] 海丽且木[2] 

机构地区：[1]新疆师范大学生命科学学院,乌鲁木齐830054 [2]新疆畜牧科学院,乌鲁木齐830000

出　　处：《四川动物》2012年第4期533-537,共5页Sichuan Journal of Zoology

基　　金：国家自然科学基金项目(30960056)

摘　　要：以分离获得的腔前卵泡数量、正常卵泡率和72h体外培养存活率为指标,比较了酶消化法、梳刮法和剪碎法3种不同方法分离塔里木马鹿Cervus elaphus yarkandensis腔前卵泡的效果。结果表明:在卵巢数目相同的情况下,以酶消化法平均获得的腔前卵泡数最多,梳刮法次之,剪碎法最少,3种不同分离方法获得腔前卵泡数量之间存在极显著差异(P<0.01);梳刮法和剪碎法分离获得的腔前卵泡正常率均极显著高于酶消化法(P<0.01);梳刮法和剪碎法分离获得的腔前卵泡在体外培养24h、48h和72h后的存活率极显著和显著高于酶消化法(培养24h、48h后,P<0.01;培养72h后,P<0.05)。由此可见,梳刮法是塔里木马鹿腔前卵泡更有效的分离方法。To identify an high-efficient method for isolation of Cervus elaphus yarkandensis preantral follicles, this paper compared the isolation effects of the comb-scrape method, the enzymatic digestion method, and the cutting method by ob- serving and recording the number of preantral follicles, the rate of normal follicles and their livability cultured for 72 hours in vitro. The results showed that the enzyme digestion method harvested significantly more preantral follicles than that of the comb-scrape and the cutting methods. However, the rate of normal follicles isolated by the comb-scrape method and cutting method was significantly higher than that of the enzymatic method. In addition, the livability of preantral follicles cultured for 24, 48, or 72 hours isolated using the comb-scrape method and the cutting method was significantly higher than that of the enzyme digestion method （24, 48 hours after culture in vitro P 〈 0.01, 72 hours after culture in vitro P 〈 0. 05 ）. The comb-scrape method is an high-efficient method for isolating preantral follicles in Cervus elaphus yarkandensis ovary.

关 键 词：塔里木马鹿 腔前卵泡 分离方法 

分 类 号：Q813[生物学—生物工程]