Caspase-3在维A酸致小鼠胚胎神经管畸形中的表达及意义  

The expression and significance of Caspase-3 in mouse with embryonic neural tube malformation induced by retinoic acid

作　　者：张艳萍 张美华 杨丹彤 张爱东 刘凯[2] 

机构地区：[1]山东省优生技术重点实验室,山东省计划生育科学技术研究所,山东济南250002 [2]山东大学医学院组织学与胚胎学教研室,山东济南250012

出　　处：《蚌埠医学院学报》2012年第6期630-632,共3页Journal of Bengbu Medical College

基　　金：山东省自然科学研究基金资助项目(2009ZRA01193)

摘　　要：目的:探讨Caspase-3在维A酸(RA)致小鼠胚胎神经管畸形发生过程中的表达及意义。方法:40只未经产昆明种小鼠随机分为实验组和对照组各4小组,每小组5只。分别在花生油及RA处理18、42、66、90 h后剖腹取胎、剥离头部神经管,Western-bloting法检测Caspase-3的表达变化。结果:Caspase-3在正常胚胎神经管组织中的表达存在时间依赖性。对照组Caspase-3在花生油灌服后18 h表达升高,42 h和66 h时表达最强,之后表达逐渐降低。实验组Caspase-3在RA处理后42 h表达水平显著低于对照组(P<0.01),66 h和90 h时表达水平均显著高于对照组(P<0.01)。结论:RA改变了昆明种小鼠胚胎神经管中Caspase-3的正常时间表达模式,这种异常的基因表达模式可能参与了RA诱导的神经管畸形发生。Objective：To explore the expression and significance of Caspase-3 in mouse with embryonic neural tube defects （NTD） induced by retinoic acid （RA）. Methods：The 40 mice were randomly divided into test and control groups. The neural tubal samples were harvested at 18,42,66 and 90 h after RA or arachis oil administration. Western blotting was used to detect the expression of Caspase-3. Results ：The expression of Caspase-3 displayed time dependence in the normal fetal neural tube tissue. In control group, the expression level of Caspase-3 increased at 18 h, and achieved the most peak at 42 and 66 h, then decreased slowly after arachis oil administration. In test group, the expression levels of Caspase-3 were significant lower than that of control group at 42 h （ P 〈 0.01 ） , and significant higher than that of normal group at 66 h and 90 h after treatment with RA （ P 〈 0.01 ）. Conclusions ： RA can change the expression pattern of Caspase-3 in embryonic neural tube of mouse, The Caspase-3 alteration may participate in the formation of NTD induced by RA.

关 键 词：神经管畸形 维A酸 CASPASE-3 凋亡 小鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R986[医药卫生—基础医学]