孕期高糖饮食对血管紧张素Ⅱ介导的成年子代大鼠肠系膜动脉的影响  

The effect of maternal high sucrose exposure on Ang Ⅱ-induced vasoconstriction of mesenteric arteries in adult offspring rats

作　　者：施爱萍[1] 李世刚[1] 徐斐朝[1] 李佳悦[1] 徐智策[1] 茅彩萍[1] 

机构地区：[1]苏州大学附属第一医院胎儿医学研究所,生殖医学中心,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2012年第3期308-313,共6页Suzhou University Journal of Medical Science

基　　金：国家自然基金重点资助项目(81030006)

摘　　要：目的研究孕期母体高糖饮食对血管紧张素Ⅱ(AngⅡ)介导的成年子代大鼠肠系膜小动脉的影响。方法以孕期母鼠给予20%蔗糖水的成年子代大鼠为高糖组(HS),孕期母鼠正常饮水的成年子代大鼠为对照组(C);分离制备成年子代大鼠离体肠系膜小动脉,检测其对AngⅡ的反应,以及环磷酰胺(CTX)对AngⅡ介导的离体血管反应的影响;同步检测成年子代大鼠离体肠系膜小动脉中,AngⅡ介导的血管张力反应(血管直径变化)与血管细胞内游离Ca2+浓度的变化;以Western-blot方法检测成年子代大鼠肠系膜小动脉组织中BKα通道蛋白的表达。结果与对照组相比,高糖组中AngⅡ介导的肠系膜小动脉的收缩效应显著增强(P<0.01);CTX可明显降低由AngⅡ介导的高糖组肠系膜小动脉的收缩增强效应(P<0.05);高糖组中由AngⅡ介导的细胞内游离Ca2+浓度显著增加(P<0.01);高糖组肠系膜小动脉组织中BKα通道蛋白的表达显著低于对照组(P<0.01)。结论孕期母体高糖饮食可对其成年子代大鼠AngⅡ介导的肠系膜小动脉的调控产生"印迹"效应。Objective To study the effect of maternal high sucrose exposure on Ang Ⅱ -induced vas- oconstriction of mesenteric arteries in adult offspring rats. Methods The pregnant Sprague-Dawley （SD） rats were randomly divided into two groups： High sucrose group（HS） rats were given 20% （W/V） su- crose solution and standard rat food, control group （C） rats were provided with fresh tap water and stan dard rat food, The mesenteric arteries from the adult offspring in both groups were used for experiments. Vascular tone induced by Ang Ⅱ in absence or presence of charybdotoxin（CTX） and Ca2＋ in single mes- enteric artery were measured in the same time. Protein abundance of large-conductance calcium activated- potassium channel（BK） α-subunits in mesenteric arteries of adult offspring rats were measured with West- ern blot. Results In HS group, Ang Ⅱ -induced contractions of the mesenteric artery were significantly increased compared with that in control group （ P 〈 0.01 ） ; Inhibition of BK with CTX significantly de- creased the Ang Ⅱ -induced maximal response （P 〈 0.05 ）. HS group displayed higher Ca2＋（ P 〈 0.01 ） and clearly decreased BK α-subunits protein expression levels, as compared with control group（P 〈 0.01 ）. Conclusion Prenatal high sucrose exposure enhance Ang Ⅱ-induced vessel tone in adult off- spring attributing to increased Ca2＋ and functional decrease of BK channels in mesenteric arteris. The ＂imprinting＂ mechanism in detail needs to be studied further.

关 键 词：孕期高糖饮食 成年子代 肠系膜小动脉 血管紧张素Ⅱ BK通道 

分 类 号：R714.5[医药卫生—妇产科学]