金荞麦提取物对IBS大鼠脊髓镇痛的干预机制  

Analgesic mechanisms of Fagopyrum cymosum extracts in rats with irritable bowel syndrome

作　　者：刘丽娜[1] 孙志广[1] 张雪梅[2,3] 周梁[4] 田超[4] 陈利[4] 邵铭[1] 史会连[1] 郭海燕[1] 

机构地区：[1]南京中医药大学第一临床医学院附属江苏省中医院,江苏省南京市210029 [2]上海市东方医院 [3]同济大学附属东方医院肿瘤科,上海市200120 [4]南京中医药大学药学院临床药理实验室,江苏省南京市210029

出　　处：《世界华人消化杂志》2012年第15期1290-1295,共6页World Chinese Journal of Digestology

基　　金：江苏省南京市科技局基金资助项目;No.201001087;江苏省中医药局科技基金资助项目;No.LZ11202;江苏省2011年度普通高校研究生科研创新计划基金资助项目;No.CXZZ2011-0779~~

摘　　要：目的:观察金荞麦(Fagopyrumcymosum,Fag)提取物对肠易激综合征(irritablebowelsyndrome,IBS)样结肠刺激(colonirritation,CI)模型内脏高敏感性的改善作用以及对动物脊髓背角内5-HT及其受体的影响.方法:采用结肠刺激新生期乳大鼠来制作IBS样CI模型.CI大鼠成年后给予口服Fag2wk,并用腹壁撤退反射(AWR)评分来评价给药后CI大鼠内脏高敏感性的变化,取大鼠脊髓用免疫组织化学法观察5-HT免疫染色,用蛋白印迹法检测5-HT1A受体(5-HT1AR)、5-HT3A受体(5-HT3AR)表达.结果:和对照组比,CI组大鼠AWR评分明显增高(20mmHg:0.625±0.518vs1.333±0.778;30mmHg:0.750±0.463vs1.667±0.888;40mmHg:1.125±0.641vs2.000±0.739;50mmHg:1.500±0.926vs2.583±0.793;60mmHg:2.125±0.991vs3.083±0.669;均P<0.05);且脊髓内5-HT染色度增加(11.250±4.833vs21.125±7.827,P<0.01),5-HT3A受体表达升高(179.038±29.786,P<0.01),5-HT1A受体则表达降低(64.523±16.873,P<0.01);高剂量Fag可降低CI大鼠AWR评分(20mmHg:0.250±0.002;30mmHg:0.875±0.044;40mmHg:1.250±0.036;50mmHg:1.875±0.050;60mmHg:2.625±0.037;均P<0.05),并下调脊髓内5-HT(13.375±5.579,P<0.05)和5-HT3AR的表达(114.200±20.983,P<0.05),上调5-HT1AR的表达(93.008±13.523,P<0.05);低剂量Fag则对CI大鼠的影响不明显.结论:Fag对IBS样CI大鼠有镇痛作用,并通过调节其脊髓内5-HT及其受体来改善痛觉过敏.AIM：To observe the effect of Fagopyrum cymosum extracts（Fag） on visceral hypersensitivity in rats with irritable bowel syndrome（IBS）-like colon irritation（CI） and to explore the possible mechanisms involved.METHODS：IBS-like models were created using neonatal CI method.CI adult rats were given Fag intragastrically for 2 wk,and visceral sensitivity was then evaluated using abdominal withdrawal re？ex（AWR） score.The expression of 5-hydroxytryptamine（5-HT） in the spinal cord was detected by immunohistochemistry and that of 5-HT1A receptor（5-HT1AR） and 5-HT3A receptor（5-HT3AR） was detected by Western blot.RESULTS：Compared to control rats,AWR scores in CI rats were significantly increased（20 mmHg：0.625 ± 0.518 vs 1.333 ± 0.778;30 mmHg：0.750 ± 0.463 vs 1.667 ± 0.888;40 mmHg：1.125 ± 0.641 vs 2.000 ± 0.739;50 mmHg：1.500 ± 0.926 vs 2.583 ± 0.793;60 mmHg：2.125 ± 0.991 vs 3.083 ± 0.669;all P 0.05）.In the spinal cord,the expression of 5-HT and 5-HT3AR was enhanced and that of 5-HT1AR expression was reduced in CI rats compared to control rats（all P 0.01）.Fag at a high dose could markedly reduce AWR scores（20 mmHg：0.250 ± 0.002;30 mmHg：0.875 ± 0.044;40 mmHg：1.250 ± 0.036;50 mmHg：1.875 ± 0.050;60 mmHg：2.625 ± 0.037;all P 0.05）,down-regulate the expression of 5-HT and 5-HT3AR,and up-regulate the expression of 5-HT1AR（all P 0.05） in spinal cord（P 0.05） in CI rats.Fag at a low dose had no significant e ect on CI rats.CONCLUSION：Fag can exert an analgesic role in IBS-like CI rats by regulating 5-HT and its receptors in the spinal cord.

关 键 词：金荞麦提取物 肠易激综合征 5-羟色胺 5-羟色胺1A受体 5-羟色胺3A受体 

分 类 号：R285.5[医药卫生—中药学]