HIF-1α、TXB2、6-Keto-PGF1α和HA联合检测在CHB肝脏微循环障碍中的诊断意义  

Significance of combined detection of HIF-1α,TXB2,6-keto-PGF1α and HA in the diagnosis of hepatic microcirculatory disturbance in patients with chronic hepatitis B

作　　者：范秀梅[1] 丁体龙[2] 代学枫[2] 于莉[2] 马勇[2] 

机构地区：[1]蚌埠医学院临床诊断学教研室,安徽省蚌埠市233000 [2]中国人民解放军第123医院南京军区肝病中心,安徽省蚌埠市233015

出　　处：《世界华人消化杂志》2012年第19期1732-1736,共5页World Chinese Journal of Digestology

基　　金：中国人民解放军南京军区医学科技创新基金资助项目;No10MA037~~

摘　　要：目的:探讨慢性乙型肝炎(chronic hepatitis B,CHB)肝脏微循环障碍时,联合检测低氧诱导因子(hypoxia-inducible factor,HIF)-1α、血栓烷素(thromboxan,TX)B2、6-酮-前列腺素F1α(6-Keto-PGF1α)及透明质酸(hyaluronic acid,HA)的临床意义.方法:275例CHB患者肝组织标本通过肝穿刺获得,同时留取外周血待检.15例正常志愿者外周血来自健康体检者.用透射电镜观察肝细胞及肝窦的超微结构变化.用化学发光法检测血清HA,放射免疫法检测血浆TXB2和6-Keto-PGF1α,免疫组织化学法标记肝穿活检标本中的HIF-1α.结果:随着CHB肝组织病变的加重,电镜示肝窦腔内红细胞聚集,肝窦阻塞和狭窄明显,狄氏腔中胶原纤维沉积及基底膜形成率逐步增多.HIF-1α在肝组织中的表达强度和范围逐渐加大,HA、TXB2逐渐上升,6-Keto-PGF1α轻度下降.结论:HIF-1α、TXB2、6-Keto-PGF1α及HA联合检测能较好地反映肝脏微循环障碍状况.AIM： To evaluate the significance of combined detection of HIF-1α, TXB2, 6-keto-PGF1α and HA in the diagnosis of hepatic microcirculatory disturbance in patients with chronic hepatitis B （CHB）. METHODS： In total, 275 patients with CHB and 15 normal volunteer were included. The ultra- structure of the liver was observed by transmis- sion electron microscopy （TEM）. HIF-1α expres- sion in liver biopsies was detected by immuno- histochemistry. Plasma levels of TXB2 and 6-keto- PGF1α were determined by chemiluminescence, and serum HA levels was measured by RIA. RESULTS： As the pathological changes of the liver were aggravated, erythrocyte aggregation, stenosis and blockage of sinus hepaticas, collagen fiber deposition, and basal membrane formation became worsened, the strength and range of ex- pression of HIF-1α was enhanced, the levels of serum HA and plasma TXB2 were raised gradu- ally, and plasma levels of 6-keto-PGFIR slightly declined. CONCLUSION： Combined detection of HIF-1α, TXB2, 6-keto-PGF1α and HA can help accurately diagnose hepatic microcirculatory disturbance in patients with CHB.

关 键 词：慢性乙型肝炎 低氧诱导因子 微循环障碍 肝窦内皮细胞 

分 类 号：R512.62[医药卫生—内科学]