长期联合应用噻吗洛尔对结膜组织炎性标记物ICAM-1和HLA-DR表达的影响  

Effect of long-term combined treatment with timolol on conjunctiva inflammatory marker ICAM-1 and HLA-DR expression

作　　者：彭坤[1] 靳隽[2] 杨玉新[1] 许银霞[3] 王保君[1] 闫义涛[1] 杨华[1] 

机构地区：[1]新乡医学院第一附属医院眼科,河南省卫辉市453100,河南省新乡市453003 [2]新乡医学院医学检验系 [3]三门峡市中心医院眼科,河南省三门峡市472000

出　　处：《眼科新进展》2012年第6期517-520,共4页Recent Advances in Ophthalmology

基　　金：新乡市科技局资助项目(编号:08S022)~~

摘　　要：目的通过观察长期应用噻码洛尔后炎性标记物细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)和人类白细胞抗原-DR(human leukocyte antigen-DR,HLA-DR)在结膜组织中的表达,探讨长期应用抗青光眼滴眼液对结膜炎症反应的影响。方法原发性开角型青光眼患者90例,根据抗青光眼药物治疗的种类分为3组:应用2种青光眼药物治疗(5g·L-1噻吗洛尔与2g·L-1阿法根)即联合用药组30例,1种抗青光眼药物治疗(5g·L-1噻吗洛尔)即单一用药组30例,无抗青光眼药物治疗即未用药组30例。另选择单纯白内障患者30例作为空白对照组。按抗青光眼药物治疗时间及白内障患病时间分为3个时相(0～6个月、7～12个月、13～18个月)组,每组10例。应用免疫组织化学染色检测结膜组织中ICAM-1和HLA-DR的表达。结果单一用药组与联合用药组在13～18个月时结膜组织中ICAM-1阳性细胞数分别是22.59±1.85和32.61±1.53,与空白对照组(5.12±1.14)相比差异均有统计学意义(均为P<0.01);联合用药组与单一用药组在13～18个月时结膜组织中ICAM-1阳性细胞数较0～6个月(6.27±1.39、6.21±1.45)、7～12个月(7.07±1.32、6.89±1.31)明显增多,差异均有显著统计学意义(均为P<0.01)。HLA-DR的表达同ICAM-1表达结果一致。结论长期联合应用抗青光眼药物可致结膜组织发生炎性反应,结膜组织中ICAM-1和HLA-DR表达明显增加。Objective To observe the expression of conjunctival inflammatory marker intercellular adhesion molecule-1 （ICAM-1） and human leukocyte antigen-DR （HLA-DR） after long-term treatment with timolol, and investigate the effect of long- term treatment with anti-glaucoma eye drops on conjunctival inflammation. Methods Ninety primary open-angle glaucoma patients were divided into three groups in accord- ance with the treatment type of anti-glaucoma drug：Combined treatment group （5 g · L^-1 timolol and 2 g ·L ^-1 alphagan,30 cases） ,single treatment group （5 g · L^-1 timolol, 30 cases） ,non-drug treatment group （30 cases）. Another 30 patients with simple cata- ract were set as control group. All patients in each group were divided into 3 time phrase sub-group （0 -6 months,7 - 12 months and 13 - 18 months） by anti-glaucoma drug treatment time and cataract during, 10 cases in each sub-group. ICAM-land HLA- DR expression in conjunctival tissue were detected by immunohistochemical method. Results At the 13 - 18 months time point, the number of ICAM-1 positive cells in con- junctival tissue in single treatment group and combined treatment group were 22.59 ± 1.85,32.61 ± 1.53, respectively, compared with that in control group （5. 12 ± 1. 14）, there were significant differences （both P 〈0.01 ）. The number of ICAM-1 positive cells in conjunctival tissue in combined treatment group and single treatment group at 13 - 18 months time point were obviously more than that at 0 - 6 months time point （6.27 ± 1.39,6.21 ± 1.45 ） and 7 - 12 months （7.07 ± 1.32,6.89 ± 1.31 ）, there were significant differences （ all P 〈 0.01 ）. HLA-DR expression was the same as ICAM-1. Conclusion Long-term combined treatment with anti-glaucoma can lead to conjunctival inflammation, ICAM-1 and HLA-DR expression in conjunctival tissue increase significantly.

关 键 词：噻吗洛尔 炎性标记物 青光眼 结膜 

分 类 号：R644[医药卫生—外科学]