小鼠组织中过氧化氢酶的活性与年龄的关系  

Age-related changes in catalase activity in different tissues of mice

作　　者：孔德胜[1] 王晓然[1] 李文君[1] 陈永春[2] 姚敏[2] 高媛[2] 

机构地区：[1]河北医科大学基础医学院 [2]河北医科大学生物化学与分子生物学教研室,石家庄050017

出　　处：《生物学杂志》2012年第3期11-13,10,共4页Journal of Biology

基　　金：河北省高等教育教学改革研究重点项目(102013);河北医科大学教育科学研究重点课题(09zd-4);河北医科大学大学生创新性实验计划经费资助项目(201012)

摘　　要：为观察小鼠组织中过氧化氢酶的活性与年龄的关系,采用高锰酸钾滴定法测定不同年龄(1、4、18月龄)小鼠肝、肾、肺、心、脾、胃、脑组织中过氧化氢酶的活性。结果显示:小鼠过氧化氢酶在不同组织中活性不同,活性高低顺序基本表现为:肝>肾>肺>心、脾、胃>脑;小鼠肺、心、脾、胃、脑各组织中过氧化氢酶的活性在1～4月龄间随年龄增加而增加,在4～18月龄间随年龄增加而降低;小鼠肝、肾组织中过氧化氢酶的活性在1～4月龄间与年龄相关性不显著,在4～18月龄间随年龄增加而降低。结果表明,小鼠肝、肾、肺、心、脾、胃、脑等组织中过氧化氢酶的活性随年龄变化而变化,机体过氧化氢酶活性的降低与机体衰老密切相关。The age-related changes in catalase activity were observed in mice liver, kidney, lung, heart, spleen, stomach and brain. The catalase activity in these tissues in mice aged of 1-month, 4-month and 18-month was determined by Permanganate Titration. The results showed that the activity of catalase varied in different tissues, in descending order as follows ： liver 〉 kidney 〉 lung 〉 heart, spleen, stomach 〉 brain. The catalase in mice lung, heart, spleen, stomach and brain showed an age-related increase in the activity from 1-month to 4-month, and an age-related decrease from 4-month to 18-month. In liver and kidney, there was no significant age-associated change in the catalase activity from 1-month to 4-month, while the activity of catalase declined with increasing age from 4- month to 18-month. The age-related change of catalase activity in mice liver, kidney, lung, heart, spleen, stomach and brain suggested that the decrease of catalase activity was closely correlated with the aging process.

关 键 词：过氧化氢酶 活性 年龄 衰老 

分 类 号：Q554.6[生物学—生物化学]