滑膜细胞[Ca^(2＋)]_i变化的理论模型研究  

A theoretical model of Caicium dynamics for fiblast-like synoviocytes

作　　者：吴怡芬[1,2] 王新宇[2] 胡芬[2] 李俊英[2] 

机构地区：[1]新疆喀什师范学院物理系,新疆喀什844006 [2]南开大学物理学院,天津300071

出　　处：《华中师范大学学报（自然科学版）》2012年第3期325-331,共7页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金项目(10747003)

摘　　要：以滑膜细胞内钙离子浓度变化为研究对象,通过分析整合影响滑膜细胞内钙离子浓度变化的模块来建立滑膜细胞[Ca2+]i变化的理论模型.先考虑参与钙调节的质膜上钙泵、内质网上钙泵和渗漏、IP3受体、Na+/K+泵、延迟整流钾通道、TRPV1通道等各个模块在网络中的作用,确定钙调控过程中各模块的数学模型.在此基础上,引入钙缓冲系统的参量,并重点突出[Ca2+]i和膜电位的相互作用,建立起滑膜细胞上胞浆[Ca2+]i变化的动力学模型.最后,调整模型参数,使得模型处于稳定的初始状态,并保证模型参数的取值在合理的范围,给出滑膜细胞响应刺激所对应的[Ca2+]i随时间变化的模拟结果,并简要分析了某些参数对模型的影响.研究结果表明:IP3受体的调控机制在辣椒素刺激滑膜细胞胞浆[Ca2+]i变化的动力学模型中有明显作用;[Ca2+]i和膜电位的相互作用对滑膜细胞钙网络调控至关重要;质膜上钙泵的密度、活性,IP3受体通道密度,TRPV1通道密度等的变化对[Ca2+]i模拟曲线形状有明显影响.In this paper,we established a calcium dynamics model of fibroblast-like synoviocytes（FLS）.A set of parameters was given for describing i changes of FLS stimulated by capsacin（CAP）.The model consists of several modules,including calcium pump in the plasma membrane,calcium pump and calcium leak in the ER,IP3 receptor,Na＋/K＋pump,the delayed rectifier potassium channel（Kv）,TRPV1 channel etc..According to the model,the interaction between i and Vm plays an important role in the i dynamics of FLS.Conclusions： It is necessary to include IP3 receptor module which contains biphasic regulation mechanism for building model.The interaction between i and Vm plays an important role in the i dynamics of FLS.Some of the physiological characteristic of FLS,such as the density of calcium pump in the plasma membrane and ER,the density of IP3R channel in ER and TRPV1 channel in the plasma membrane,the activity of ER calcium leak related protein,the concentration of calcium buffering proteins etc.have impacts on the shape of the calcium dynamic curves.

关 键 词：滑膜细胞 模块 [CA2+]I 膜电位 数学模型 

分 类 号：Q67[生物学—生物物理学]