湿地内外滇池水体中微囊藻毒素对小鼠生殖毒性的研究  

Study on Reproductive Toxicity of Microcystin in Peripheral and Internal Wetland of Dianchi Lake

作　　者：沈放[1] 路斌[1] 石自博[1] 杨黎江[1] 

机构地区：[1]昆明学院生命科学与技术系,云南昆明650214

出　　处：《昆明学院学报》2012年第3期33-36,共4页Journal of Kunming University

基　　金：云南省教育厅科研基金资助项目(09C0133);昆明学院科研课题资助项目(2009L017)

摘　　要：通过提取湿地内外滇池水体水样中的微囊藻毒素,检测湿地内外水体中微囊藻毒素对小鼠的生殖毒性,以及对其体外和体内精子活性的影响.结果表明:湿地外周滇池水体中的微囊藻毒素对小鼠具有生殖毒性,特别在精子的生成和精子活性方面表现出较强的毒性作用;湿地内水体的生殖毒性明显低于湿地外水体,对小鼠的睾丸和附睾不会形成器质性变化,也不会影响精子的数量和精子活性(与对照组比较P>0.05).结果证实湿地能有效抑制滇池水体中微囊藻毒素的形成,对滇池水质可起到有效的净化作用.Microcystins was extracted in water of peripheral and internal wetland of Dianchi Lake.Reproductive toxicity of microcystins in mice and the influence of microcystins on sperm motility in vitro and in vivo were examined.The microcystins from peripheral wetland in Dianchi Lake has reproductive toxicity in mice and especially shows stronger toxic effects in the production and activity of sperm.Reproductive toxicity of the water sample from internal wetland is significantly lower than that of peripheral wetland causing none pathological changes in mouse testis and epididymis,and no impact on sperm numbers and sperm activity（compared with the control group,P〉 0.05）.The results demonstrate that wetland can effectively inhibit the production of microcystin and effectively purify the water quality in Dianchi Lake.

关 键 词：滇池 湿地 微囊藻毒素 生殖毒性 精子 精子活性 

分 类 号：X52[环境科学与工程—环境工程] R994.6[医药卫生—毒理学]