腹膜透析液添加尿激酶对腹透患者氧化应激及内皮功能的影响  

The effects of Peritoneal dialysate add urokinase on the oxidative stress and endothelial function in patients with peritoneal dialysis SOD, MDA. the plasma endothelin and nitric oxide in patients with Chronic Kidney Disease（ Ⅳ ）

作　　者：张清德[1] 曲忠森[2] 

机构地区：[1]菏泽医学专科学校内科教研室,菏泽274030 [2]上海市第六人民医院内科,上海200233

出　　处：《临床肾脏病杂志》2012年第6期252-255,共4页Journal Of Clinical Nephrology

基　　金：山东省高等学校科技计划项目（J11LF78）

摘　　要：目的探讨腹膜透析液添加尿激酶对腹透患者血清超氧化物歧化酶（SOD）、丙二醛（MDA）及血浆内皮素（endothelin，ET）、一氧化氮（nitricoxide，NO）的影响。方法将60例慢性肾脏病（CKD4期）患者随机分为治疗组（30例）和对照组（30例），两组基础治疗相同，治疗组在腹膜透析液中添加尿激酶，治疗4周后观察两组患者SOD、MDA、ET、NO及临床症状的变化。用比色法测定健康对照组、对照组、治疗组血清MDA和SOD水平，用放射免疫法测定测定ET的变化，NO采用硝酸还原法测定。结果与健康对照组比较，对照组、治疗组血清SOD活性降低（P〈0．05），NO升高（P〈0．05），MDA含量升高（P〈0．05），ET水平升高（P〈0．01）。对照组虽能够降低ET水平和NO，但未见SOD、MDA的变化，治疗组能够回升SOD活性，降低MDA含量，与健康对照组及对照组有明显差别（P〈0．05），与对照组比较，治疗组在降低ET和NO方面疗效更为显著（P〈0．01）。结论腹膜透析液添加尿激酶可通过降低氧化应激反应，改善血管内皮功能，降低ET和NO水平，对CKD患者有治疗作用。Objective To explore the effects of Peritoneal dialysate add urokinase on the serum Superoxide Dismutase（SOD）, Malondialdehyde（MDA）, plasma endothelin and nitric oxide in patients with chronic kidney diease, （CKD）（ IV ）. Methods Sixty patients with CKD（ Ⅳ ） were divided into experimental group （n = 30） and control group （n = 30）randomly,and basal treatment was given to both groups. The experi-mental group was treated with Peritoneal dialysate add urokinase, Changes of Superoxide Dismutase, Malondialdehyde,plasma ehdothelin, nitric oxide, proteinuria and clinical symptom were observed after administration of the medicine for 4 weeks, The levels of SOD and MDA were measured by colorimetry, the alterations of plasma endothelin（ET） were measured by radiation immu nological test and nitric Oxide （NO） were measured by nitrate reduction method in control group, experimental group and huangkui Capsule treatment Peritoneal dialysate add urokinase group. Results Compared with control group, the activity of SOD and nitric Oxide were decreased while the content of MDA was increased in experimental group and treatment group, moreover, the level of plasma endothelin was increased in experimental group and treatment group. （P〈0. 01）, the elevated plasma endothelin was decreased in experimental group with nimodipine（P〈0. 01）, but no obvious changs of SOD and MDA. The low activity of SOD and nitric Oxide were decreased（P〈0. 05） while the big content of MDA was decreased（P〈0. 05）, and lower level of plasma endothelin was induced（P〈0. 05） in Peritoneal dialysate add urokinase, treatment group as compared with experimental group and control group（P〈0. 05, P〈0. 01）. Conclusions Peritoneal dialysis solution adding urokinase may decrease the oxidative stress reaction, improve endothelial function, have a role in the treatment of chronic kidney disease （IV）. patients Peritoneal dialysate add urokinase improves the blood volume by lowering the reaction of oxi

关 键 词：腹膜透析液 尿激酶 内皮素 一氧化氮 超氧化物歧化酶 丙二醛 

分 类 号：R459.5[医药卫生—治疗学]