伤寒沙门菌PFGE分子分型及耐药性研究  

PFGE molecular typing and antimicrobial resistance of Salmonella typhi

作　　者：林一曼[1] 李迎慧[1] 扈庆华[1] 吕东月[1] 邱亚群[1] 石晓路[1] 

机构地区：[1]深圳市疾病预防控制中心,广东深圳518055

出　　处：《热带医学杂志》2012年第6期750-753,共4页Journal of Tropical Medicine

基　　金：国家科技重大专项(2008ZX10004-006);深圳市科技计划项目(201102107;201102103)

摘　　要：目的对深圳市2004-2011年伤寒沙门菌进行脉冲场凝胶电泳(PFGE)分子分型及耐药性分析,为临床用药和追踪传染源提供依据。方法采用K-B法对42株伤寒沙门菌进行20种抗菌药物敏感性测试,并用PFGE对其进行分子分型。结果 42株伤寒沙门菌株对氨苄西林、阿莫西林/克拉维酸、氨苄西林/舒巴坦、头孢噻吩、头孢他啶、头孢曲松、头孢吡肟、头孢西丁、阿米卡星、庆大霉素、卡那霉素、环丙沙星、左旋氧氟沙星、复方新诺明、甲氧苄啶、氯霉素和四环素17种药物的敏感率均超过90%,对萘啶酸耐药率最高达61.5%。42株伤寒沙门菌株可分为SZ0001-SZ0028共28个PFGE型别,其中流行优势型为SZ0023型别。结论结合药物敏感试验结果和PFGE分子分型结果可以判断伤寒疫情的优势株型。Objective To analyze the PFGE typing and antimicrobial resistance of Salmonella typhi in Shenzhen in 2004- 2011, and provide the evidence for guiding rational use of antibiotics and tracking the infection source. Method Antibiotic sensitivity tests of 20 kinds of antibiotics were performed by the K-B method, and genotyping were carried out by PFGE method. Results The sensitive rates of 17 antibiotics, AMP, AMX, SAM, CEF, CAZ, CRO, FEP, FOX, AMK, GEN, KAN, CIP, LEV, SXT, TMP, CL and TFT were over 90%. The highest resistant rate was NAL, which was 61.5%. There were 28 different molecular types among 42 strains of Salmonella typhi and the dominant type was type 23. Conclusion Clinicians can treat typhoid fever and prevent the spread of Salmonella typhoid with suitable antibiotics according to the results of antibiotic sensitivity and PFGE typing.

关 键 词：伤寒沙门菌 药敏 PFGE分型 优势型 

分 类 号：R378.2[医药卫生—病原生物学]