广州汉族配偶人类血小板抗原基因分型的调查  

A study of genotyping for human platelet antigen in Guangzhou han couples

作　　者：周豪杰[1] 杨承东 聂咏梅[1] 

机构地区：[1]广州血液中心,广东广州510095 [2]白云区妇幼保健院,广东广州510400

出　　处：《热带医学杂志》2012年第6期757-760,共4页Journal of Tropical Medicine

基　　金：广州市医药卫生科技项目(2009-YB-230)

摘　　要：目的调查配偶之间人类血小板抗原(HPA)的不配合率并评估其在新生儿同种免疫性血小板减少症(NAIT)中的作用。方法采用SSP法对200对广州汉族配偶进行了HPA-1～-16基因分型。结果配偶双方HPA-1～-6及HPA-15均在同一HPA位点具有不同基因型,HPA-7～-14及HPA-16均为a/a纯合子。得到HPA-1～-16的等位基因频率,HPA-15、HPA-3、HPA-2、HPA-6、HPA-5、HPA-1和HPA-4的不配合率为别为37.42%、37.02%、8.02%、3.84%、2.44%、0.75%和0.24%,其余HPA位点的不配合率为0。结论 HPA-5可能是广州汉族人群NAIT最重要的一个HPA系统,HPA-15、HPA-3、HPA-2、HPA-6和HPA-4也分别具有其免疫学意义,这为HPA的抗体检测以及NAIT的诊断治疗提供了实验依据。Objective To investigate the mismatch probability of the human platelet antigen （HPA） between couples and evaluate its role in neonatal alloimmune thrombocytopenia（NAIT）. Method A total of 200 couples of han nationality in Guangzhou were genotyped for HPA-lto -16 by PCR-SSP. Results Gene frequencies of 200 couples for HPA-1 to -16 were obtained. The mismatch probabilities for HPA-15, HPA-3, HPA-2, HPA-6, HPA-5, HPA-1 and HPA-4 were 37.42%, 37.02%, 8.02%, 3.84%, 2.44%, 0.75% and 0.24%, respectively, and it was zero for the other HPA sites. Conclusion HPA-5 may be the most important HPA system for NAIT in Guangzhou Han population. HPA-15, HPA-3, HPA-2, HPA-6 and HPA-4 may also have their immunological role. This study provides a basis for screening for HPA antibodies and also improves the diagnosis and treatment of NA1T.

关 键 词：人类血小板抗原 基因分型 PCR-SSP 不配合率 

分 类 号：R558.2[医药卫生—血液循环系统疾病]