蕨麻正丁醇提取物对心肌缺血／再灌注损伤大鼠肿瘤坏死因子-α水平的影响  

The effects of PotentiUa anserine L. extract on level of tumor necrosis factor- α in rats with acute myocardial ischemia/reperfusion injury

作　　者：王震[1,2] 李建军[3] 董化江[4] 单云官[4] 王永阔[4] 李伯森[4] 

机构地区：[1]天津医科大学研究生院,天津300071 [2]武警后勤学院附属医院医疗科,天津300162 [3]武警后勤学院附属医院脑系科中心,天津300162 [4]武警后勤学院解剖学教研室及民政部遗体防腐与整形整容重点实验室,天津300162

出　　处：《中国中西医结合急救杂志》2012年第3期141-143,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家“十一五”科技支撑计划项目（2007BAK38805）

摘　　要：目的探讨蕨麻正丁醇提取物对心肌缺血／再灌注（I／R）损伤大鼠肿瘤坏死因子-α（TNF-α）的影响。方法50只Wistar大鼠按随机数字表法分为假手术组、模型组和蕨麻提取物高、中、低浓度预处理组，每组10只。右心房取血，用酶联免疫吸附试验（ELISA）检测血清TNF-α含量，用逆转录-聚合酶链反应（RT-PCR）、蛋白质免疫印迹法（Westernblotting）检测心肌组织中TNF-α mRNA和蛋白表达。结果与假手术组比较，模型组及蕨麻提取物中、低浓度预处理组血清TNF-α水平及心肌组织TNF-α mRNA[吸光度（A）值]和蛋白表达均明显升高，以模型组升高更显著[血清TNF-α（pg/L）：93．8±5．3比46．5±4．2，心肌组织TNF-α mRNA：0．79±0．11比0．37±0．10，心肌组织TNF-α蛋白：0．92±0．09比0．49±0．11，均P〈0．05]；与模型组比较，蕨麻提取物高、中、低浓度预处理组TNF-α均明显降低，以蕨麻提取物高浓度预处理组降低更显著（血清TNF-α：44．0±2．9，心肌组织TNF-α mRNA：0．33±0．09，心肌组织TNF-α蛋白：0．45±0．18，均P〈0．05）。结论TNF-d在大鼠心肌I／R损伤过程中表达上调，用蕨麻正丁醇提取物进行干预可降低TNF-α的表达。Objective To investigate the effects of Potentilla anserine L. （Pc） extract on the level of tumor necrosis factor-α（TNF-α ） in rats with acute myocardial isehemia/reperfusion （I/R） injury. Methods Fifty Wistar rats were randomly divided into sham operation group, model group and Pa high-dose group, middle-dose group and low-dose group. Right atrial blood was collected to detect the serum TNF-α content by enzyme-linked immunosorbent assay （ELISA）, and the expressions of TNF-α mRNA and protein of the tissues were determined by reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting. Results Compared with the sham operation group, the expressions of the absorbance （A） value of TNF-α mRNA in serum and myocardial tissues in model group, Pa high-dose group, middle-dose group and low-dose group were increased significantly, and the most significant one was the model group [ serum （μg/L） ： 93.8±5.3 vs. 46.5±4.2, tissue mRNA ： 0.79±0.11 vs. 0.37±0.10, tissue protein ： 0.92±0.09 vs. 0.49±0.11, all P〈0.05]. Compared with the model group, TNF-α reduced significantly in Pa high-dose group, middle-dose group and low-dose group, and the most significant one was the high-dose group （serum ： 44.0±2.9, tissue mRNA ： 0.33±0.09, tissue protein： 0.45±0.18, all P〈 0.05）. Conclusions TNF-ot is up-regulated in rats with acute myocardial I/R injury. Pa extract can be used to intervene and reduce the expression of TNF-α.

关 键 词：蕨麻正丁醇 大鼠 缺血/再灌注损伤 心肌 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学] R256.2[医药卫生—中医学]