多指标综合评价法优选湖北海棠叶中总黄酮提取工艺  

Optimization of Extraction Technology for Total Flavonoids from Malus hupehensis by Multi-index Comprehensive Evaluation Method

作　　者：王幸[1] 师延琼[1] 覃鸿恩[1] 郭东艳[1] 

机构地区：[1]陕西中医学院,陕西咸阳712046

出　　处：《中国实验方剂学杂志》2012年第13期46-49,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：陕西省教育厅重点实验室项目(11JS036);陕西省重点学科专项资金项目

摘　　要：目的:优选湖北海棠中总黄酮的提取工艺。方法:以总黄酮及根皮苷含量为指标,采用单因素试验法筛选提取方法和提取次数;以浸膏得率,总黄酮及根皮苷含量为综合评价指标,对主要影响因素乙醇体积分数、溶剂用量、提取时间进行正交试验优选。结果:最佳提取工艺为8倍量70%乙醇回流提取3次,每次1 h。结论:优选工艺稳定可行,可作为湖北海棠中总黄酮的提取工艺。Objective： To optimize extraction technology of total flavonoids from Malus hupehensis. Method： Single factor test was made to select extraction method and extraction times with the content of total flavonoids and phloridzin as indexes; With the content of extract yield, total flavonoids and phloridzin as comprehensive evaluation index, main factors were optimized by orthogonal test which including the concentration of ethanol, the amount of solvent and extraction time. Result： Optimum extraction technology was： refluxing extracted 3 times with 8 folds the amount of 70% ethanol, 1 h each time. Conclusion： Optimized process was stable and feasible, and it could be extraction process of total flavonoids from M. hupehensis.

关 键 词：湖北海棠 正交设计 总黄酮 综合评价 根皮苷 

分 类 号：R283.6[医药卫生—中药学]