HPLC测定宁夏枸杞中东莨菪素和东莨菪苷含量  

Determination of Scopoletin and Scopolin in Lycium barbarum by HPLC

作　　者：徐飞[1,2] 王晓中[1] 张雷[1] 龚波林[1] 

机构地区：[1]宁夏大学能源化工重点实验室,银川750021 [2]宁夏疾病预防控制中心,银川750004

出　　处：《中国实验方剂学杂志》2012年第13期67-70,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划子项目(2009BAI72B00);宁夏回族自治区科技攻关计划项目;宁夏大学科学研究基金(ZR1144)

摘　　要：目的:采用高效液相色谱法,建立宁夏枸杞中东莨菪素和东莨菪苷的含量测定方法。方法:色谱条件,DiamonsilC8柱(4.6 mm×150 mm,5μm);流动相A为乙腈-水-醋酸(5∶94.5∶0.5),流动相B为乙腈-水-醋酸(72∶27.5∶0.5),梯度洗脱。流速1 mL·min-1,柱温30℃,检测波长346 nm。结果:东莨菪素在0.1～10 mg·L-1线性关系良好,回归方程Y=32 568X+637.8(r2=0.999 9),平均回收率98.2%,RSD 1.45%;东莨菪苷在0.1～10 mg·L-1线性关系良好,回归方程Y=23 951X+746.7(r2=0.999 9),平均回收率101.6%,RSD 1.27%。结论:该方法准确可靠,可用于测定宁夏枸杞中东莨菪素和东莨菪苷的含量,为宁夏枸杞开发利用提供理论依据。Objective： An HPLC method was established for determination of scopoletin and scopolin in Lycium barbarum. Method： The chromatographic separation was performed on Diamonsil Cs column （4.6 mm× 150 mm, 5 μm）, acetonitrile-water-acetic acid （5：94.5： 0.5） as mobile phase A and aeetonitrile-water-acetic acid （72： 27.5：0.5 ） as mobile phase B with gradient elution. The flow rate was 1 mL . min^-1 at 30 ℃, and the detection wavelength was set at 346 nm. Result： The calibration curve was linear in the range of 0.1-10 mg . L^-1 for scopoletin （r =0. 999 9） , and the regression equation was Y = 32 568X ＋ 637.8. The recovery was 98.2% , with RSD of 1.45%. The calibration curve was linear in the range of 0. 1-10 mg . L^-1 for scopolin （r =0. 999 9） , and the regression equation was Y = 23 951 X ＋ 746.7. The recovery was 101.6% , with RSD of 1.27%. Conclusion： The method is accurate and reliable, which could he used as a quality control method for seopoletin and scopolin in L. barbarum, and provide basis for development and utilization of L. barbarum.

关 键 词：宁夏枸杞 东莨菪素 东莨菪苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]