基于代谢组学技术的黄连解毒汤配伍规律研究  

Study on the Compatibility Rule of Huanglian Jiedu Tang Based on Metabonomics

作　　者：柳长凤[1] 陈平平[1] 刘树民[1] 方圆[1] 包顺茹[1] 张翀[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《中国实验方剂学杂志》2012年第13期146-152,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)(2007CB512608)

摘　　要：目的:研究黄连解毒汤组方中君、臣、佐、使药的配伍规律。方法:取雄性Wistar大鼠54只,分为空白组、模型组、黄连组、黄连-黄芩组、黄连-黄芩-黄柏组、黄连解毒汤组;模型组和各给药组于大鼠背部皮下注射2,4-二硝基苯酚(25 mg·kg-1大鼠体质量)造模;各给药组于造模前30 min ig给予黄连、黄连-黄芩、黄连-黄芩-黄柏、黄连解毒汤1次(分别为7.2,12,16.8,24 g生药/kg大鼠体质量);收集造模后1,3,5,7,13,22 h各时间点的尿液,尿液样品进UPLC-TOF/MS检测分析,质谱数据采用MarkerLynx XS软件统计分析,并采用主成分分析法(PCA)和正交偏最小二乘-判别分析法(OPLS-DA)对获得的数据进行处理。结果:在得分图中,黄连组与模型组混为一组,黄连-黄芩组与模型组较接近,离空白组较远,黄连-黄芩-黄柏组与模型和空白组显著聚类区组,并且与空白组较接近,黄连解毒汤组与模型组和空白组显著聚类区组,与它们的距离相当;同时黄连、黄连-黄芩、黄连-黄芩-黄柏和黄连解毒汤各给药组分别对33,15,36,40个生物标志物具有干预作用,综合评价各实验组对热病证候的整体代谢和生物标志物的干预作用,结果黄连解毒汤全方作用最好,黄连-黄芩-黄柏次之,黄连作用第三,黄连-黄芩的干预作用最弱。结论:黄连作为方中君药发挥主要的药理作用,黄柏和栀子作为佐使药也发挥了其各自的作用,但黄芩作为臣药的地位有待商榷。Objective： To study the compatibility rule of Huanglian Jiedu Tang based on metabonomics. Method： Fifty-four male Wistar rats were randomly divided into six groups including control group, model group, Huanglian group, Huanglian-Huangqin group, Huanglian-Huangqin-Huangbai group .and Huanglian Jiedu Tang group. The treatment groups were orally administrated with corresponding drug at a dose of 7.2, 12, 16. 8, 24 g crude drug. kg^-1, respectively. Thirty minute later, the model group and treatment groups were injected with a DNP solution （sc, 25 mg.kg^-1）. The urine of rats was collected during period of 0-1, 1-3, 3-5, 5-7, 7-13, 13- 22 h, respectively. The urine samples were analyzed by UPLC-TOF/MS and the urine mass spectrometry data were processed by MarkerLynx XS software and analyzed further by principal component analysis （PCA） and orthogonalpartial least squares-discriminant analysis （OPLS-DA）. Result： In the score plot, the Huanglian group was mixed with model group, the Huanglian-Huangqin group was close to model and farther away from the control group, the Huanglian-huangqin-huangbai group, the model and conrtol group were distributed in 3 clusters in the principal components analysis diagram without overlap, and closer to the control group, the Huanglian Jiedu Tang group, the model group and control group were distributed in 3 clusters in the principal components analysis diagram without overlap, and their distances were identical; meanwhile, 33, 15, 36, 40 biomarkers were respectively interfered by the Huanglian, Huanglian-Huangqin, Huanglian-Huangqin-Huangbai and Huanglian Jiedu Tang groups. After comprehensively evaluating the intervention effect of treatment groups according to the overall metabolism of fever syndrome and endogenous biomarker variations, we found that the order of effect strength was Huanglian Jiedu Tang, Huanglian-Huangqin-Huangbai, Huanglian, Huanglian-Huangqin. Conclusion： Huanglian as the monarch herb play a major pharmacological action; Huangbai and Zhizi as

关 键 词：黄连解毒汤 配伍规律 代谢组学 

分 类 号：R285.5[医药卫生—中药学]