大黄蛰虫丸对异丙肾上腺素诱导大鼠心肌纤维化的影响  

Effect of Dahuang Zhechong Pill on Myocardial Fibrosis Induced by Isoprenaline in Rats

作　　者：宋金燕[1] 刘俊田[1] 庞晓明[1] 毛俊俊[1] 王淑跃[1] 吴迪[1] 

机构地区：[1]西安交通大学医学院药理学系,西安710061

出　　处：《中国实验方剂学杂志》2012年第13期169-172,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30572347)

摘　　要：目的:探讨大黄蛰虫丸抗心肌纤维化作用及其初步机制。方法:50只SD大鼠随机分为正常对照组,模型对照组,阳性对照组,大黄蛰虫丸高、低剂量组。除正常对照组外,其他4组大鼠背部皮下注射异丙肾上腺素(Iso)5 mg·kg-1,连续10 d,建立大鼠心肌纤维化模型。造模同时阳性对照组按照每日1 g·kg-1剂量灌胃给予丹参,大黄蛰虫丸高、低剂量组分别按照每日3.2,1.6 g·kg-1剂量灌胃给药,每天1次,连续28 d。正常对照组和模型对照组给予等体积的生理盐水。观察大黄蛰虫丸对大鼠心脏指数,心肌病理学变化,心肌组织中羟脯氨酸(Hyp)含量,超氧化物歧化酶(SOD)活性,丙二醛(MDA)含量等的影响。结果:与正常对照组比较,模型对照组心脏指数增加,心肌组织出现明显的胶原纤维增生,心肌组织Hyp含量明显增高,胶原容积积分(CVF)与血管周围胶原面积/血管腔横断面积(PVCA)显著升高(P<0.01),SOD活性下降,MDA含量增高(P<0.05)。与模型对照组相比,大黄蛰虫丸高、低剂量组心肌纤维化病变减轻,心肌组织Hyp含量下降,CVF,PVCA减小(P<0.05或P<0.01),SOD活性与MDA含量无明显变化。结论:大黄蛰虫丸能够抑制心肌纤维化的发生发展,逆转心肌重构,但该作用可能与抗脂质过氧化作用无关。Objective： To study the protective effects of Dahuang Zhechong Pill （DHZCP） on myocardial fibrosis induced by isoprenaline （Iso） in rats. Method ： Fifty SD rats were divided into 5 groups randomly ： normal control, model control, positive control （ Danshen, 1 g. kg^- 1. d^ - 1 ） , low and high doses of DHZCP （ 1.6, 3.2 g. kg^-1.d^-1）. Myocardial fibrosis model was estabiished by subcutaneous injection of 5 mg-kg^-1 Iso in rats for 10 days except normal control rats. The drugs were administered intragastrally to rats for 28 days. The histopathological change was examined, and level of hydroxyproline and malondialdehyde （MDA） as well as superoxide dismutase （SOD） activity were determined in the myocardial tissue. Result： The results showed that heart index, level of hydroxyproline and MDA, collagen volume fraction （CVF）, perivascular collagen area （PVCA） were increased, and SOD activity was reduced in myocardial tissue of model rats in comparison with normal rats. The histopathological examination showed that there was a significant collogen proliferation and myocardial fibrosis. However, DHZCP improved and reversed the changes induced by Iso. Conclusion： DHZCP is able to reverse the myocardial fibrosis induced by Iso and to improve ventricular remodeling in rats, which may not related to antioxidative effect.

关 键 词：大黄蛰虫丸 心肌纤维化 异丙肾上腺素 羟脯氨酸 

分 类 号：R285.5[医药卫生—中药学]