运脾止泻方治疗脾虚腹泻的机制研究  

The Antidiarrheal Mechanism of Yunpi Zhixie Formulas

作　　者：郭健[1] 赵淑英[1] 席时芳[1] 倪磊[1] 杨向竹[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国实验方剂学杂志》2012年第13期189-192,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：留学归国科研启动基金项目(39批)

摘　　要：目的:观察运脾止泻方治疗脾虚腹泻的作用机制。方法:将大鼠随机分为3组:空白对照组(蒸馏水)、利血平造模组和运脾止泻方组。以利血平0.2 mg·kg-1ip 3 d造模之后,模型组和药物组分别ig蒸馏水和运脾止泻冲剂(0.6 g·kg-1)连续6 d。观察血清D-木糖、胃蛋白酶、肠胃动素(MTL)、肠血管活性肠肽(Vip)和乙酰胆碱酯酶(AChE)以及小肠微绒毛的变化。结果:造模后大鼠D-木糖、胃蛋白酶、Vip和AChE水平降低,MTL升高,小肠微绒毛破损严重。而运脾止泻方组大鼠D-木糖水平[(500±68)mg·L-1]高于模型组和空白对照组[(275±28),(419±57)mg·L-1];胃蛋白酶活性[(0.63±0.25)U·mg-1]明显高于模型组[(0.25±0.06)U·mg-1],MTL活性[(25.49±1.9)U·mg-1]明显低于模型组[(151.43±9.7)U·mg-1]。Vip活性[(106.25±15.83)ng·L-1]与空白对照组[(113.00±8.25)ng·L-1]接近,AChE活性[(2.36±0.09)U·mg-1]高于模型组[(1.15±0.03)U·mg-1],但仍然低于空白对照组[(3.25±0.09)U·mg-1];小肠微绒毛明显修复。结论:以温中运脾为治则的运脾止泻方能通过促进蛋白质的消化、促进小肠吸收以及减缓胃肠运动而抑制腹泻,且具有明显的保护小肠微绒毛的作用。Objective ： To observe （smoothing spleen to stop diarrhea formulas）. group （distilled water）, drug （Yunpi Zhixie the mechanism of antidiarrheal effect of Yunpi Zhixie formulas Method： Rats were randomly divided into three groups： control formulas） group and model group （reserpine）. Reserpine wasadministrated to rats consequent 6 days, of drug and model group by intraperitoneal injection for 3 days in a prophylactic manner. In distilled water was administrated to model group, while Yunpi Zhixie formulas were administrated to drug group. The changes in D-xylose, pepsin, motilitin （MTL） , vasoactive intestinal peptide （Vip）, acetylcholinesterase （ACHE） and microvilli were observed. Result： After reserpine was administrated, the level of the D-xylose, pepsin, AChE and Vip decreased, MTL increased. Microvilli were disturbed severely. After Yunpi Zhixie formulas was administrated, the level of D-xylose [ （500 ± 68） mg.L^-1 ] is higher than those in model and control group [ （275±28）, （419 ±57） mg.L^-1]. Pepsin [ （0.63 ±0.25） U.mg^-1] is higher than that in model group [ （0. 25 ± 0. 06） U.mg^-1 ] obviously, MTL [ （0. 25 ± 0.06） U. mg^-1 ] was lower than that in model group [ （151.43 ±9.7） U-mg^-1 ]. Vip [ （106.25 ± 15.83） ng.L^-1 ] was back to control group＇s level [ （113.00±8.25） ng.L^-1]. AChE [ （2.36±0.09） U.mg^-1] was higher than model group [ （1.15± 0.03） U.mg^-1＇], but still lower than that in control group [ （3.25 ±0.09） U.mg^-1] .The microvilli were almostly repaired. Conclusion： Yunpi Zhixie formulas by Wang Peng-fei under the direction of warming middle to invigorate spleen inhibits diarrhea through promoting digestion, absorption, slowing motility of gastrointestinal tract and provides a protection for microvilli.

关 键 词：运脾止泻方 脾虚 腹泻 利血平 

分 类 号：R285.5[医药卫生—中药学]