荔枝核总黄酮对肝纤维化大鼠肝组织MMP-2表达的影响  

Effect of Total Flavone from Litchi chinensis Sonn on Expression of MMP-2 in Rats Livers with Hepatic Fibrosis

作　　者：欧士钰[1] 罗伟生[1] 靳雅玲[1] 覃浩[1] 孙旭锐[1] 

机构地区：[1]桂林医学院附属医院消化内科,广西桂林541004

出　　处：《中国实验方剂学杂志》2012年第13期209-213,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西卫生厅重点课题(2010052)

摘　　要：目的:观察基质金属酶-2(MMP-2)在实验性肝纤维化大鼠组织中的表达,探讨荔枝核总黄酮(total flavone from Litchi chinensis Sonn,TFL)抗肝纤维化的作用机制。方法:大鼠随机分为正常组,模型组,TFL高,低剂量组及秋水仙碱阳性对照组。模型组,TFL治疗组及秋水仙碱阳性对照组以二甲基亚硝胺腹腔注射4周制作大鼠肝纤维化模型;造模同时TFL高、低剂量组及秋水仙碱分别以TFL(200,100 mg·kg-1·d-1),秋水仙碱(colchicine,col)灌胃给药,正常组及模型组给予等体积生理盐水灌胃,6周后处死大鼠,抽取下腔静脉血检测谷草转氨酶(AST),谷丙转氨酶(ALT),取肝脏同一部位行HE,Masson染色观察大鼠肝纤维化程度,采用免疫组化检测各组肝组织MMP-2的表达。结果:TFL高、低剂量组和正常组血清AST,ALT均明显低于模型组,具有显著性差异(P<0.05)。与模型组比较TFL治疗组可明显抑制MMP-2的表达(P<0.05),改善大鼠肝纤维化程度(P<0.05)。结论:荔枝核总黄酮可减轻肝损伤及改善实验性大鼠肝纤维化程度,抑制MMP-2的表达可能是其抗肝纤维化作用的机制之一。Objective： To observe theexpression of matrix metalloproteinase-2 （MMP-2） in the livers of rats with hepatic fibrosis, and the effects of total flavone from Litchi chinensis Sonn （TFL） on rats. Method： The rat model of hepatic fibrosis was established by intraperitoneal injection of DMN （dimethylnitrosamine）. TFL was orally given at a dose of 100, 200 mg.kg^-1 .d^-1. Colchicine was used as a positive control. After 6 weeks, all rats were sacrificed and their left liver lobes were examined histopathologically. The degree of fibrosis was evaluated by HE and Masson straining; serum levels of alanine transaminase （ALT） and aspartate aminotransferase （AST） were detected by an biochemical analytical tool in the laboratory test department of our hospital. The expression of MMP- 2 in liver tissue was assessed by S-P immunohistochemical staining using polyclonal antibodies against MMP- 2. Result： Serum levels of ALT Mad AST were significantly lower in the TFL group and the colchicine group than that in the fibrosis model group （ all P 〈 0.05）. The degree of hepatic fibrosis in the TFL group was less severe compared with those of the fibrosis model. The expression levels of MMP-2 in the TFL group and colchicine group were lower than in that the liver fibrosis model group （P 〈 0.05）. Conclusion： TEL can improve the degree of fibrosis, the mechanism possibly involve the inhibition of the expression of MMP-2.

关 键 词：荔枝核总黄酮 肝纤维化 基质金属酶-2 

分 类 号：R285.5[医药卫生—中药学]