高效液相色谱波长切换法测定菝葜有效部位中4种指标成分的含量  

HPLC wavelength switching simultaneous determination of the contents for 4 kinds of index components in the active ingredients of Smilax china L.

作　　者：谢长[1] 沈婵娟[1] 廖鹏程[1] 陈树和[1,2] 邹鹏程 叶晓川[1] 刘焱文[1] 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室,湖北武汉430061 [2]湖北省中医院药学部,湖北武汉430061 [3]湖北人福药业股份有限公司,湖北咸宁437000

出　　处：《中国医院药学杂志》2012年第12期929-932,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金项目(编号:30870256)

摘　　要：目的:建立HPLC波长切换法同谱测定菝葜有效部位中落新妇苷、黄杞苷、白藜芦醇和槲皮素4种指标成分的含量测定方法。方法:采用高效液相色谱法,Kromasil 100-5C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,流速:1.0mL.min-1,柱温30℃,多波长切换时间序列采样:0～45 min为290 nm;45～57 min为303 nm;57～80 min为370 nm。结果:落新妇苷在0.037～0.370μg、黄杞苷在0.011～0.110μg、白藜芦醇在0.302～3.020μg和槲皮素在0.042～0.420μg范围内与峰面积呈良好的线性关系(n=6);4种成分的平均加样回收率分别为97.32%,97.55%,98.33%,97.12%;RSD分别为1.7%,2.1%,1.5%,1.9%。结论:本法简便、准确,所测结果稳定、重现性好,可用于菝葜有效部位的质量控制。OBJECFIVE To establish an HPLC method of wavelength switching simultaneous determination of the contents for astilbin, engelitin, resveratrol, quercetin 4 kinds of index components in the aqueous extracts of Smilax china L. METHODS The analysis was carried out on Kromasil Cls （4. 6 mm×250mm, 5μm） column. The mobile phase was composed of acetoni- trile and 0. 1% phosphoric acid with gradient elution and the flow rate was set at 1.0 mL. rain 1, and the column temperature was 30℃ ,The detection wavelengths：0 - 45 min,290 nm; 45 - 57 rain, 303 nm; 57 - 80 rain, 370 nm RESULTS The linear ranges of astilbin, engelitin, resveratrol, quercetin were 0. 037 - 0. 370, 0. 011 - 0. 110, 0. 302 - 3. 020, 0. 042 - 0. 420μg, re- spectively. The average recoveries were 97. 32%,97. 55%,98. 33%,97. 12%. RSD were 1.7% ,2. 1%,1.5%, 1.9%, respec- tively. CONCLUSION The developed method is rapid, accurate with high repeatability and stability,which is helpful to control the quality of the active ingredients of Smilax china L.

关 键 词：菝葜有效部位 落新妇苷 黄杞苷 白藜芦醇 槲皮素 波长切换法 

分 类 号：R927.2[医药卫生—药学]