壳聚糖-聚乙烯醇复合凝胶基质抑菌性能的研究  

Bacteriostatic effect of chitosan-polyving alcohol gel matrix

作　　者：李微微[1,2] 程岚[2] 于炜婷[1] 赵姗[1] 宋慧一[1,3] 吕国军[1] 谢威扬[1] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023 [2]辽宁中医药大学,大连116600 [3]中国科学院研究生院,北京100049

出　　处：《中国新药杂志》2012年第12期1407-1409,共3页Chinese Journal of New Drugs

基　　金：国家自然科学基金(10979050)

摘　　要：目的:制备壳聚糖-聚乙烯醇复合凝胶基质,考察不同相对分子质量,不同浓度的壳聚糖对复合凝胶的抗菌抑菌性能的影响。方法:采用一剂量法对不同相对分子质量不同浓度的壳聚糖凝胶、壳聚糖-聚乙烯醇复合凝胶对大肠杆菌的抑菌效果进行比较。结果:随壳聚糖浓度增大对大肠杆菌抑菌效果逐渐增强,随壳聚糖相对分子质量的减小抑菌效果增强,但相对分子质量小于5 000时无抑菌圈。结论:明确了壳聚糖相对分子质量和浓度对壳聚糖-聚乙烯醇复合凝胶抗菌抑菌作用的影响。确定壳聚糖-聚乙烯醇复合凝胶中壳聚糖适宜的相对分子质量为60 000、浓度为2%。壳聚糖作为一种新的药用材料与聚乙烯醇合用对大肠杆菌具有良好的抑菌作用,为新药开发奠定良好基础。Objective： To prepare chitosan-polyvinyl alcohol gel matrix,and test the antimicrobial activity of chitosan with different molecular weights at various concentrations.Methods： The bacteriostatic effects of chitosan gel of different molecular weights at various concentrations and chitosan-polyvinyl alcohol gel were compared by one dosage technique using E.coli as the test microbial.Results： The antimicrobial activity against E.coli was strengthened with increasing concentrations of chitosan.In contrast,the antimicrolical activity against E.coli became weaker with increasing molecular weights of chitosan.The gel with molecular weight less than 5 000 had no antimicrobial effect.Conclusion： We determined the effects of the concentration and molecular weight of chitosan in chitosan-polyvinyl alcohol gel.The optimal concentration and molecular weight of chitosan in chitosan-polyvinyl alcohol gel are 60 000 and 2%.Chitosan,as a new kind of medicinal materials,combined with polyvinyl alcohol has an antimicrobial effect against E.coli,providing a reference for the development of new drugs.

关 键 词：壳聚糖 聚乙烯醇 凝胶 大肠杆菌 抗菌 相对分子质量 浓度 

分 类 号：R96[医药卫生—药理学]