一株致面部感染的灰黄青霉的形态学观察  

Morphological Observation on A Strain of Penicillium griseofulvum Causing Facial Infection

作　　者：张静[1] 黄怀球[2] 袁立燕[2] 薛汝增[3] 张晓辉[3] 钟毅[2] 赵静[2] 李美荣[2] 

机构地区：[1]中山大学孙逸仙纪念医院皮肤性病科,广东广州510120 [2]中山大学附属第三医院皮肤性病科,广东广州510630 [3]广东省皮肤性病防治中心,广东广州510091

出　　处：《皮肤性病诊疗学杂志》2012年第3期128-131,共4页Journal of Diagnosis and Therapy on Dermato-venereology

摘　　要：目的:明确一株致面部感染的灰黄青霉的形态学特征。方法:使用SDA培养基对灰黄青霉进行大培养,观察其菌落特征;使用玻片法对灰黄青霉进行小培养,制作扫描电镜样本后观察其微观形态特征。结果:此株灰黄青霉的菌落呈灰绿色至深褐色,接种后5天菌落直径为1～1.5 cm,表面可见大量的放射状皱纹,中心有脐状突起;质地呈绒状,带轻微絮状,在边缘面上的颗粒状通常更为突出;边缘可见淡黄色色素环;菌落反面呈乳黄色,质地平滑,表面可见放射状皱褶。扫描电镜显示分生孢子梗长,孢梗茎表面粗糙。帚状枝复杂,瓶梗呈瓶状,梗基顶端无膨大,分生孢子链较长,排列成圆柱状,可弯曲,分生孢子呈圆形或宽椭圆形,孢壁粗糙。结论:此株灰黄青霉的形态特征与野生株基本相符。Objective：The objective of this study was to determine the morphological characteristics of a strain of Pennicillium griseofulvum causing facial infection. Methods： The strain of Pennicillium griseofulvum was inoculated on SDA medium and the colony characteristics were investigated. The microcuhure was adopted by slide method and its micromorphological characteristics were observed by the scanning electron microscope. Results：The colonies were 1 - 1.5 cm in diameter on the 5th day after inoculating and the color was gray-green to dark brown. There were radial wrinkles and umbilical-like protrusions on the surface. The texture of colonies was velvet-like with a slight flocculent and granular at the edge. Pale yellow pigment ring was visible around the colonies. Reverse side of colonies was milky yellow and had a smooth surface with radial folds： This strain of Pennicillium griseofulvum had long eonidiophores and complicated broom sticks. Its phialide was ampulliform and the top of metula was not expanded. Conidial chain was long and arranged in a close cylinder form with conidia showing subglobose or broadly elliptical. Both conidiophores and conidial had rough surfaces. Conclusion： The morphological characteristics of this Pennicillium griseofulvum are consistent with the wild strain.

关 键 词：灰黄青霉 感染 面部 形态学 

分 类 号：R379[医药卫生—病原生物学]