白念珠菌伊曲康唑耐药性与乳酸脱氢酶抗体结合水平的关系研究  

Relationship between Itraconazole Resistance of Candida albicans and Cross-reactive Degree with Anti-LDH Antibody

作　　者：钟毅[1] 黄怀球[1] 张静[2] 张晓辉[3] 赵静[1] 李美荣[1] 

机构地区：[1]中山大学附属第三医院皮肤性病科,广东广州510630 [2]中山大学孙逸仙纪念医院皮肤性病科,广东广州510120 [3]广东省皮肤性病防治中心,广东广州510095

出　　处：《皮肤性病诊疗学杂志》2012年第3期132-134,共3页Journal of Diagnosis and Therapy on Dermato-venereology

摘　　要：目的:探讨白念珠菌与乳酸脱氢酶(lactate dehydrogenase,LDH)抗体的结合水平与伊曲康唑耐药性的关系。方法:实验用白念珠菌临床株共30株,包括伊曲康唑耐药株、中介株和敏感株各10株,采用P0013B放射免疫沉淀分析裂解液提取白念珠菌总蛋白,应用ELISA法与LDH抗体反应,检测并比较三组菌株与LDH抗体结合的水平。结果:ELISA检测耐药株与LDH抗体相对结合量为348.71±34.36,中介株与LDH抗体相对结合量为327.00±85.60,敏感株与LDH抗体相对结合量为443.43±35.64,三组差异有统计学意义(F=8.52,P=0.003)。结论:白念珠菌耐药株与LDH抗体相对结合量较敏感菌株低,提示白念珠菌与LDH抗体相对结合量下降可能与菌株耐药的发生有关。Objective：To explore the relationship between itraconazole resistance of Candida albicans and cross-reactive degree with anti-LDH antibody. Methods：The whole intracellular proteins of Candida albicans were extracted by P0013B RIPA lysis buffer from 30 isolates （ including ten strains resistant to itraconazole, ten medium strains and ten sensitive strains）, respectively followed to react with anti-LDH antibody by ELISA, then the combination degree of 3 groups of strains were measured and analysised. Results：The relative combination degree with anti-LDH antibody was 348.71 ± 34. 36 for resistant Candiada albicans isolates, and it was significantly lower than that for sensitive isolates （443.43 ±35.64） and medium isolates （ 327.00 ± 85.60 ; F = 8.52, P = 0. 003 ）. Conclusion：The occurrence of itraconazole resistance in Candida albicans may be relatated with the decrease of combination degree of anti-LDH antibody.

关 键 词：白念珠菌 乳酸脱氢酶 苹果酸脱氢酶 耐药 

分 类 号：R379.4[医药卫生—病原生物学]