脂质运载蛋白2及其受体与子痫前期发病的关系  

Correlation of lipocalin-2 and its receptor expressions with preeclampsia

作　　者：曹文红[1] 陈维萍[2] 李静[1] 叶元华[1] 

机构地区：[1]青岛大学医学院附属医院产科,266003 [2]青岛大学医学院附属医院妇科,266003

出　　处：《中华妇产科杂志》2012年第6期418-421,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的研究脂质运载蛋白2（LCN-2）及其受体（NGALR）与子痫前期发病的关系。方法选择2010年12月至2011年4月在青岛大学医学院附属医院住院分娩的轻度子痫前期孕妇26例（轻度子痫前期组），重度子痫前期孕妇38例（重度子痫前期组）；以同期正常晚期妊娠妇女25例为对照组。采用ELISA方法检测各组孕妇血清LCN-2水平，采用逆转录PCR技术检测各组孕妇胎盘组织中LCN-2、NGALRmRNA的表达水平，采用蛋白印迹法检测胎盘组织中LCN-2、NGALR蛋白的表达水平。结果（1）轻度及重度子痫前期组孕妇血清LCN-2水平分别为（58±20）、（90±18）μg／L，均明显高于对照组的（19±6）μg/L，分别比较，差异均有统计学意义（P〈0．01）；重度子痫前期组明显高于轻度子痫前期组，两组比较，差异有统计学意义（P〈0．01）。（2）轻度及重度子痫前期组孕妇胎盘组织中LCN-2mRNA表达水平分别为0．55±0．14、0．61±0．14，均明显高于对照组（0．28±0．16），分别比较，差异均有统计学意义（P〈0．01）；轻度及重度子痫前期组孕妇胎盘组织中LCN-2蛋白表达水平分别为2．2±0．4、2．4±0．5，均明显高于对照组的1．4±0．4，分别比较，差异均有统计学意义（P〈0．01）。（3）轻度及重度子痫前期组孕妇胎盘组织中NGALRmRNA表达水平分别为0．46±0．11、0．46±0．14，对照组为0．45±0．15，分别比较，差异均无统计学意义（P〉0．05）；轻度及重度子痫前期组孕妇胎盘组织中NGALR蛋白表达水平分别为2．7±0．8、3．0±0．9，对照组为2．7±0．9，分别比较，差异无统计学意义（P〉0．05）。（4）轻度及重度子痫前期组孕妇血清LCN-2水平与胎盘组织LCN-2mRNA、蛋白的表达水平及收缩压、舒张压均无明显相关性（P〉0．05），与24h尿蛋白总量及血尿酸水平有相关性（r=0．565、0．476，P〈0．01）。结论血�Objective To research the correlation of the expressions of lipocalin-2 （LCN-2） and its receptor （NGALR） in serum and placenta with preeclampsia. Methods From Dec. 2010 to Apr. 2011, 64 women with preeclampsia who delivered in Affiliated Hospital of Qingdao University Medical College were recruited in the study, including 26 women with moderate preeclampsia （MPE group） and 38 women with severe preeclampsia （SPE group）. Twenty-five healthy pregnant women were taken as control group. LCN-2 and NGALR mRNA and protein expression in placenta were measured by reverse transeription-PCR （RT- PCR） and western blot, respectively. Results （ 1 ） The serum levels of LCN-2 in MPE group and SPE group [ （58 ±20）, （90± 18） μg/L] were significantly higher than that in control group [ （ 19 ±6） μg/L, P 〈 0. 01 1 ; the serum LCN-2 level in SPE group was significantly higher than that in MPE group （ P 〈 0. 01）. （2） LCN-2 mRNA expression in placenta in MPE group and SPE group （0.55 ±0. 14, 0. 61 ± 0. 14） were both significantly higher than that in control group （0. 28 ±0. 16, P 〈0. 01 ） ; LCN-2 protein expression in placenta of MPE group and SPE group （ 2.2 ± 0. 4, 2.4 ± 0. 5 ） were also significantly higher than that in control group （1. 4 ±0. 4, P 〈0. 01 ） , no significant difference was found between MPE groupand SPE group （ P 〉 0. 05 ）. （ 3 ） No significant difference was found in the expressions of NGALR mRNA in placenta among MPE group, SPE group and control group （0. 46 ± 0. 11, 0.46 ± 0. 14, 0.45 ± 0. 15, P 〉 0. 05 ）. （4） NGALR protein expressions in MPE group, SPE group and control group were 2.7 ±0. 8, 3.0 ± 0. 9, and 2. 7 ± 0. 9, and there were no significant difference among these three groups （P 〉 0. 05）. （5） In preeclampsia, serum LCN-2 level significant associated with 24 hours total urinary protein and uric acid （ r = 0. 565, 0. 476, P 〈0. 01 ）. LCN-2 serum level were not associated with systolic pres

关 键 词：先兆子痫 脂笼蛋白质类 受体 肽 胎盘 急相蛋白质类 原癌基因蛋白 质类 

分 类 号：R714.2[医药卫生—妇产科学]