湖州地区乙肝疫苗免疫失败儿童乙肝病毒S基因“a”决定簇变异分析  

Variations of hepatitis B virus S gene ＂a＂ determinant among children with failure of immunization in Huzhou city

作　　者：钱福初[1] 邹伟华[1] 秦基取[1] 戴利成[1] 

机构地区：[1]浙江省湖州市中心医院,湖州313000

出　　处：《中华实验和临床病毒学杂志》2012年第3期176-178,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解湖州地区乙肝疫苗免疫失败儿童病毒S基因型变异情况。方法应用聚合酶链反应（PCR）方法对29例HBV—DNA阳性乙肝疫苗免疫失败儿童乙肝病毒S基因进行扩增，PCR扩增产物进行序列分析。结果29例样本中测序结果显示，B基因型26例，c基因型3例，有9例样本在s区“a”决定簇发生变异，变异主要存在“a”决定簇内127、129、131、133、141、142、144等氨基酸位点。结论湖州地区乙肝疫苗免疫失败儿童在“a”决定簇区确实存在变异，可能是发生免疫失败的原因，但并未形成明显优势变异株。Objective To study the variants of S gene of hepatitis B virus among the breakthrough infection children immunized with hepatitis B vaccine in Huzhou city. Methods HBV DNA S gene regions were amplified and sequenced in 29 children with HBsAg-positive/HBV DNA-positive after immunized with hepatitis B vaccine. Results The sequencing results showed that 26 cases were B genotypes, 3 were C genotypes. The amino acids mutations were presented in 9 children serum HBV DNA. The major amino acid mutations were in the ＂a＂ determinant of HBV DNA S gene. Conclusion Different S gene variants maybe cause HBV vaccination failure in some children in Huzhou city. No predominant variant was found.

关 键 词：肝炎病毒 乙型 儿童 保健服务 变异(遗传学) 基因 

分 类 号：R7[医药卫生—临床医学] R1