Sabin IPV基础免疫后不同时间的中和抗体水平研究  

Study on neutralizing antibody titer in different time intervals after immunization with Sabin inactivated poliovirus vaccine

作　　者：刘婧[1] 马艳[1] 孙明波[1] 伍胤杰[1] 周武刚[1] 谢炳锋 张名[1] 唐聪[1] 杨卉娟[1] 杨晓蕾[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,云南省重大疾病传染病疫苗研发重点实验室,昆明650118

出　　处：《中华实验和临床病毒学杂志》2012年第3期196-198,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家“重大新药创制”科技重大专项（2012ZX09101318）;云南省科技创新强省计划（2007AD015）作者单位：通信作者：杨卉娟,Email：[e-mail];杨晓蕾,Email：[e-mail]

摘　　要：目的探讨不同剂量配比的Sabin株脊髓灰质炎灭活疫苗（SabinIPV）基础免疫后中和抗体的持续时间及加强免疫前后中和抗体水平的变化。方法用不同配比的Ⅰ、Ⅲ型脊髓灰质炎病毒原液制备两批SabinIPV，并使用GSK制备的DTaP—WIPV作为阳性对照组，对18只Wistar大鼠进行3针基础免疫后，每间隔3个月采血直到加强免疫前，并同时于加强免疫后1个月采血，并对血清中抗脊髓灰质炎病毒3个型别的中和抗体效价进行初步研究。结果大鼠采用0、1、2月免疫程序进行3针免疫后，Ⅰ、Ⅱ、Ⅲ型脊髓灰质炎病毒中和抗体的几何平均滴度随着时间的变化均有所下降，但绝大部分组大鼠的血清中和抗体阳转率仍维持在100％，在加强免疫后，各组的三个型别的中和抗体水平在短期内明显升高。结论SabinIPV有良好的免疫持久性，并在加强免疫后，可产生更高水平的中和抗体。Objective To study on persistence and pre- or post-booster immunization of neutralizing antibody level which is induced by different concentration of Sabin inactivated poliovirus vaccine（IPV） after three-dose immunization. Methods Two batches of Sabin IPV were produced using different concentration of type 1 and type 3 poliovirus. Together with DTaP-w IPV （boostrixTM-poliO, GSK, Belgium） as control group. Serum samples were collected every three months until pre-booster immunization and one month after booster immunization was also assembled. Neutralizing antibody titers against three types poliovirus were determined using micro-neutralization test. Results After immunization,the GMTs（ geometric mean titers） of neutralizing antibodies against three types poliovirus reduced over time,but the seropositivity rates of most groups of rats were remained at 100%. All the groups after booster immunization increased significantly in a very short peroid of time and could induced high level neutralizing antibody against poliovirus in rats. Conclusion Sabin IPV had a good immune persistence, and could induced a higher level neutralization antibody after booster immunization.

关 键 词：脊髓灰质炎病毒疫苗 灭活 抗体 病毒 

分 类 号：R186[医药卫生—流行病学]