木豆毛状根的诱导及其培养条件的研究  

Induction and Culture Condition of Hairy Roots of Cajanus cajan(L.) Millsp.

作　　者：于海娣[1] 王慧梅[1] 付玉杰[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2012年第4期481-483,500,共4页Bulletin of Botanical Research

基　　金：948引进项目(异性木豆内生真菌生产木豆酸技术引进;2012-4-06)

摘　　要：利用发根农杆菌LBA9402对木豆叶片直接进行诱导产生毛状根。本实验研究出诱导木豆毛状根的最佳条件是,以木豆叶片为外植体,于1/2MS固体培养基上预培养2～4 d,菌液浓度OD600=0.6～0.8,浸染20 min,共培养3 d,诱导率为60.00%。在分子水平用PCR检测表明,发根农杆菌9402Ri质粒上的T-DNA成功整合进木豆毛状根的基因组中。Hairy roots of Cajanus cajan （L.） Millsp. was induced by Agrobacterium rhizogenes LBA9402 with direct inoculation. The optimum condition of induction of hairy roots in this test was as follows, the leaves of C. cajan were used as explants to be inoculated on 1/2MS solid medium, pre-culture for 2 d, the concentration of bacterium was OD = 0.6 -0.8, dissemination for 20 min, co-culture for 3 d, the rate of the hairy roots was 60%. PCR analysis at molecular level proved that T-DNA of Ri plasmid of A. rhizogenes 9402 has been successfully transferred into the genome of hairy roots of C. cajan.

关 键 词：木豆 发根农杆菌 毛状根 PCR检测 

分 类 号：Q945[生物学—植物学]