慢性阻塞性肺疾病大鼠支气管上皮MRP1 mRNA及蛋白水平变化及化痰降气方逆转的作用  

Expression of multidrug resistance-associated protein 1 in bronchial epithelium of models rat with chronic obstructive pulmonary disease and the reversal effects of Huatanjiangqi Capsule on it

作　　者：汪电雷[1] 张弦[1] 陶秀华[1] 曹银[1] 彭波[2] 李泽庚[1] 

机构地区：[1]安徽中医学院 [2]安徽中医学院第一附属医院

出　　处：《中国临床药理学与治疗学》2012年第6期621-626,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金(81001592);教育部科学技术重点项目资助(210101);安徽高校省级自然科学研究重点项目资助(KJ2010A210)

摘　　要：目的:研究慢性阻塞性肺疾病(COPD)大鼠支气管上皮MRP1mRNA及蛋白水平变化及化痰降气方逆转的作用。方法:采用烟熏复合木瓜蛋白酶雾化吸入法制备COPD大鼠模型,将造模后的大鼠随机分为治疗组和模型组,另以正常大鼠作为对照组。治疗组予以化痰降气方(生药5.8g/kg)i.g.治疗给药,2次/d,连续15d后,分别采用RT-PCR、Western blot技术检测各组大鼠肺支气管上皮MRP1mRNA及蛋白水平的表达。结果:化痰降气方治疗能明显改善COPD模型大鼠的肺功能;COPD模型组MRP1mRNA及蛋白水平较正常对照组显著降低(P<0.01);与COPD模型组比较,化痰降气方治疗组MRP1mRNA及蛋白水平显著升高(P<0.01)。结论:化痰降气方能逆转COPD状态下MRP1mRNA及蛋白水平表达的降低,这可能是其治疗COPD作用机制之一。AIM： To investigate the effects of Huatanjiangqi Capsule on the expression of multidrug resistance-associated protein 1 in the lung tissues of models rat with Chronic obstructive pulmonary disease （COPD）. METHODS： COPD rat model was established by using smoke exposure composite papain aerosol inhalation. COPD rats were randomly divided into model group and Huatanjiangqi treatment group, and normal rats were as controls. Fifteen days after administration Huatanjiangqi, lung tissues were taken and expressions of MRP1 were determined by RT- PCR and Western blot. RESULTS： Huatanjiangqi Capsule could improve the lung function of COPD models rat. The MRP1 mRNA and pro-tein expression in COPD model group was significantly lower than those in control group （P〈 0.01）. Huatanjiangqi could up-regulate the MRP1 mRNA and protein expression compared with COPD model group （P〈0.01）. CONCLUSION： Huatanjiangqi Capsule could up-regulate the expression of MRP1 in the lung tissues of COPD models rat. This is probably the mecha- nism of Huatanjiangqi Capsule in treatment COPD.

关 键 词：COPD MRP1 表达 化痰降气方 逆转作用 

分 类 号：R965[医药卫生—药理学]