外源性血管内皮衍生超极化因子对脑缺血再灌注损伤的影响  

Effects of exogenous endothelium-dependent hyperpolarizing factor on cerebral ischemia reperfusion injury

作　　者：焦传安[1,2] 宋标[2,3] 陈志武[2] 

机构地区：[1]巢湖市第一人民医院精神科 [2]安徽医科大学药理学教研室 [3]合肥市第三人民医院药学部

出　　处：《中国临床药理学与治疗学》2012年第6期648-653,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(81173596);安徽省自然科学基金项目(11040606M196)资助

摘　　要：目的:硫化氢(hydrogen sulfide,H2S)为一种假定的血管内皮衍生超极化因子(endothelium-derived hyperpolarizing factor,EDHF),本研究探讨外源性H2S,即外源性假定的EDHF对脑缺血再灌注损伤的影响。方法:采用线栓法复制大鼠局灶性脑缺血(MCAO)再灌注损伤模型,测定动物行为功能、脑组织梗死体积、脑组织含水量、血清乳酸脱氢酶(LDH)活性及丙二醛(MDA)含量,用HE染色法观察脑组织学改变。结果:H2S供体硫氢化钠(NaHS,i.v.)0.195、0.390、0.780mg/kg能明显改善神经功能状态,降低缺血再灌注后脑梗死体积百分比,降低脑含水量,显著地抑制局灶性脑缺血再灌注损伤大鼠血清MDA含量和LDH活性,并不同程度地改善大鼠脑病理组织学的变化。结论:NaHS可明显改善大鼠脑缺血再灌注性损伤,提示外源性ED-HF(H2S)有抗脑缺血再灌损伤作用。AIM. Hydrogen sulfide （H2S） is one of the supposed endothelium-dependent hyperpolarizing factor （EDHF）, the protective effects of exogenous H2 S, namely exogenous supposed EDHF on cerebral ischemia reperfu- sion （I/R） injury was observed in present study. METHODS： Sodium hydrosulfide （NariS） was taken as a donor of He S. Focal cerebral I/R injury was caused by occluding the right middle cerebral artery （MCAO） in rats. The numerical data, which were from infarct volume, neurological deficit, brain water content, malondial- dehyde （MDA） level and lactate dehydrogenase （LDH） activities in serum, were evaluated. HE staining was used to observe brain pathologic changes. RESULTS：0. 195, 0. 390, 0. 780 mg/kg Naris markedly ameliorated the neurological deficit, decreased the percentage of infarct vol- ume, reduced the brain water content, and dra- matically inhibited the cerebral I/R injurer-in- duced increases of LDH activity and MDA con- tent in serum, improved brain pathologic chan- ges in different degrees. CONCLUSION. NariS significantly improved the cerebral I/R injury in rats, this suggests exogenous supposed EDHF （H2S） has a significant protective effect against cerebral I/R injury.

关 键 词：内皮依赖性超极化因子 硫化氢 硫氢化钠 脑缺血再灌注损伤 

分 类 号：R965.2[医药卫生—药理学]