骨髓间充质干细胞对放射性回肠损伤的病理观察  

Effect of Bone Marrow Stem Cells on Repairment of Ileum Radiation Damage

作　　者：宋祥福[1] 孙冰雪[2] 张静春[3] 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,长春130021 [2]吉林大学生命科学院药物制剂,长春130021 [3]吉林大学中日联谊医院检验科,长春130031

出　　处：《实验动物与比较医学》2012年第3期191-194,共4页Laboratory Animal and Comparative Medicine

基　　金：[基金项目】吉林省科技厅项目资助课题（20080446）

摘　　要：目的观察骨髓间充质干细胞（MSCs）在电离辐射诱发的空肠组织损伤中的迁徙、定居及修复作用。方法大鼠5GyX线全腹部照射，每隔72h照射1次，共5次，制备放射性回肠损伤动物模型。大鼠随机分成正常对照组、模型组及MSCs治疗组，在激光共聚焦显微镜下观察MSCs在回肠组织中富集情况。病理学观察MSCs对放射性回肠损伤的修复作用。结果MSCs经尾静脉注入2d即富集回肠组织内，7d后广泛分布在肠系膜血管周围。正常大鼠回肠黏膜结构清楚，模型组黏膜上皮细胞坏死脱落，以3d较重，累及全层细胞变性：治疗组的黏膜较相应的模型组厚，7d肠黏膜坏死较模型组轻。结论MSCs可以迅速地富集在放射性损伤的回肠组织中，促进回肠再生与修复。Objective To observe the migration, colonization and repairing effects of marrow mesenchymal stem cells （MSCs） on ileum damage induced by radiation in rats. Methods The model of ileum radiation damage was made. Four rats from the ileum radiation damage model were sacrificed at 2 and 7d respectively after they were injected mesenchymal stem cells 1 ml （concentration： 1 × 10^6/ml）, and ileal tissue were taken out, then the enrichment of MSCs in the ileum were observed under confocal microscope. Thirty-six rats were randomly divided into normal control group, model, and MSCs group （n=12）. Animals in each group after the last injection of MSCs were sacrificed at 3 and 7 d, pathological changes of the ileum was observed. Results The occurrence of MSCs was observed in the ileum 2 d after MSC infusion, and widely distributed around the mesenteric vessels 7 d later. The intestinal structure was clear in normal control group, and epithelial cell was significantly necrosis at 3 d, involving the whole layer of cell degeneration in model group; compared with the model group, mucosal get thickness, and intestinal necrosis was relieved 7 d in treatment group. Conclusion The MSCs can be rapidly enriched in ileum radiation damage and promote regeneration and repairment.

关 键 词：骨髓间充质干细胞 电离辐射 回肠 组织修复 

分 类 号：R818[医药卫生—放射医学]