APPswe／PS1dE9转基因小鼠繁殖性能及生长发育观察  

Analysis on Reproductive Performance and Growth of APPswe/PSldE9 Transgenic Mice Model for Alzheimer＇s Disease

作　　者：李娜[1] 王维[1] 黎陈静[1] 张颖妍[1] 张晟[1] 章海燕[1] 张周[1] 

机构地区：[1]中国科学院上海药物研究所,上海201203

出　　处：《实验动物与比较医学》2012年第3期222-227,共6页Laboratory Animal and Comparative Medicine

基　　金：【基金项目]中国科学院战略生物资源技术支撑体系专项（CZBZX一1）,重大新药创制国家科技重大专项（2009ZX09301-001）

摘　　要：目的为建立阿尔茨海默病APPswe／PS1dE9转基因小鼠生物学特性标准化资料提供实验依据。方法APPswe／PS1dE9转基因小鼠杂合子交配繁殖，建立基因型鉴定方法，选择160窝次的1158只小鼠分别统计4胎内不同胎次、不同基因型的繁殖性能及生长发育指标。结果①APP／Ps1片段大小为600bp；②第2胎和第3胎的仔鼠数量及阳性数量均大于第1胎（P〈0．05），阳性率则无明显差异（P〉0．05）；③第1、2胎之间的胎间隔天数较短（P〈0．05），第2、3胎的窝产仔数、离乳仔数及离乳率均高于其它两胎（P〈0．05）；④第3胎和第4胎的初生窝重、泌乳力和离乳个体重较为稳定；⑤雌雄APP／PS1小鼠的最高体重分别为24．45±0．92g和30．21士2．53g；⑥该转基因小鼠的基因型不影响其体重（P〉O．05）。结论建立了APP／PS1小鼠的基础生物学特性资料；Objective To afford experimental basis for establishing the standard biological properties＇ datum of APPswe/PS 1 dE9 transgenic mice model on Alzheimer＇s disease. Methods The heterozygote of the APP/PS 1 transgenic mice were used to breed, and the identify method of genotype was established. The reproductive performance and growth of different litters and genotype were analysed within four litters of 1158 mice in 160 litters. Results （1） The section of APP/PS1 was 600 bp. （2） The quantity of the newborn mice and transgenic positive in litter 2 and 3-were more than litter l（P〈0.05）, but the positive rate had no significant difference（P〉0.05）. （3） The birth interval between parity 1 and 2 was shorter than other＇s （P〈0.05）. Litter size, number of mice weaned and survival rate after weaning of the parity 2 and 3 were higher than other two （P〈0.05）. （4） The litter weight at birth, lactescence, body weight after weaning of parity 3 and 4 were stable. （5） The highest weight of female and male APP/PS 1 transgenic mice are 24.45 ± 0.92 g and 30.21 ±2.53 g separately. （6） This transgenic mouse genotype did not affect its weight. Conclusions The basic biological characteristics data of APP/PS 1 transgenic mouse are established, and provide the exact data for scientific research.

关 键 词：阿尔茨海默病 APP/PS1转基因小鼠 繁殖性能 生长发育 

分 类 号：Q95-33[生物学—动物学]