国产氨舒钠体外抗菌活性  

In vitro antibacterial activities of ampicillin/sulbactam

作　　者：雷军[1] 凌保东[1] 张淑华 洪峥[3] 吴文蓉 王浴生[3] 

机构地区：[1]川北医学院药理学教研室,四川南充637007 [2]四川抗生素工业研究所,成都610051 [3]华西医科大学,成都610047

出　　处：《川北医学院学报》2000年第1期1-4,共4页Journal of North Sichuan Medical College

基　　金：四川省教委重点科研课题

摘　　要：目的 :比较国产氨舒钠 (ASN)和进口优立新 (ULX)制剂 (均由氨苄西林 (AMP)与舒马坦 (SBT)以 2 :1比例组成 )的体外抗菌活性。方法 :采用平皿二倍稀释法 ,多点接种仪点种细菌测定最小抑菌浓度 (MIC) ,并观察药物的MBC及接种菌量、pH、血清浓度改变对药物抗菌作用的影响。结果 :AMP与ASN或ULX对革兰阳性菌的MIC50 之平均比值分别为 4 8和 5 1;对革兰阴性菌的平均MIC50 比值分别为 6 3和 6 6 ;ASN和AMP的MBC为 2～ 4×MIC。接种菌量在 10 3 ～ 10 9CFU/ml、pH值在 5～ 9及血清浓度在 0 %～ 2 0 %的范围内 ,药物的MIC仅提高 1～ 4倍。结论 :SBT能增强氨苄西林的抗菌活性 ;接种菌量、pH、血清浓度改变对药物抗菌活性无明显影响。Aim:To compare the antibacterial activities of domestic and imported ampicillin(AMP)/sulbactam(SBT)preparatin,and also to study the influence of pH of broth,inoculum size,and various comcemtrations of serum in broth on amtibacterial activity of the antibiotics.Methods:The collection of 510 clinical isolates in 1991～1992 Chengdu was used to assess in vitro susceptibility to antibiotics by using two fold plate or broth dilution method.Results:the MIC 50 value of the two prepartions was about 5 times and over 6 times that of AMP against gram positive bacteria and gram negative bacteria,respectively.In the comditions of changes of pH(from 5 to 9),inoculum size(from 10 3 秋10 9 CFU/ml),and serum concentrations(from 0% to 20%)in broth,the MIC value increased 1～4.Conclusion:SBT could augment the activities of AMP against bacteria.The changes of pH,inoculum size,and serum concentions of in broth omlp slightly influence the antibacterial activities of AMP/sbt preparation.

关 键 词：Β-内酰胺酶 氨舒钠 体外抗菌活性 

分 类 号：R978.11[医药卫生—药品]